This lectin is a 150,000 molecular weight tetrameric glycoprotein consisting of four disulfide-linked chains, two of about 36,000D and two of about 38,000D. Sambucus nigra lectin binds preferentially to sialic acid attached to terminal galactose in (a-2,6), and to a lesser degree, (a-2,3), linkage. Binding is also inhibited to some extent by lactose or galactose. This lectin does not appear to bind sialic acid linked to N-acetylgalacto-samine. SNA has been reported to inhibit cell-free protein synthesis.||Applications: |Suitable for use in ELISA. Other applications not tested.||Recommended Dilutions:|Optimal dilutions to be determined by the researcher.||Sugar Specificity: |Sialic Acid||Activity;|≥85%||Storage and Stability:|Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile 10mM HEPES, pH 8.5, 0.1mM Ca++. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.