SK-BR-3 cells
Cat# C0006007
Size : OneFrozenvial
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Disease:
Breast adenocarcinoma Pleural effusion Homo sapiens Breast; mammary gland Epithelial Adherent This is a hypertriploid to hypotetraploid human cell line(+A, +B, +C, +E, +F, +G, -D) with abnormalities including dicentrics, acrocentric fragments, rings, secondary constrictions, large metacentrics or polycentrics and large submetacentric marker Female, Caucasian, 43 yrs of age McCoy's 5a (Gibco 16600082) + 10% FBS 1:2 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37 °C Complete culture medium supplemented with 5% (v/v) DMSO I Bacteria: Negative Yeast: Negative Mycoplasma: Negative HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Amelogenin: X CSF1PO: 12 D13S317: 11,12 D16S539: 9 D5S818: 9,12 D7S820: 9,12 THO1: 8,9 TPOX: 8,11 vWA: 17 If use of this culture results in a scientific publication, it should be cited in the publication as: SK-BR-3 (AddexBio C0006007) 4T1 AU-565 BT-20 BT-474 BT-483 BT-549 JIMT-1 MCF-7 SK-BR-3 T47D ZR-75-1 Origin:
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Protein Expression:
HER2/c-erb-2 Isoenzymes:
AK-1, 1-2; ES-D, 1; G6PD, B; GLO-I, 2; PGM1, 1-2; PGM3, 1 DNA Profile (STR):
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References for SK-BR-3 cells:
1. Brockhoff, G., Heckel, B., Schmidt-Bruecken, E., Plander, M., Hofstaedter, F., Vollmann, A., and Diermeier, S. Differential impact of Cetuximab, Pertuzumab and Trastuzumab on BT474 and SK-BR-3 breast cancer cell proliferation. Cell Prolif, 40: 488-507, 2007.
2. Choi, E. J. and Kim, G. H. Apigenin causes G(2)/M arrest associated with the modulation of p21(Cip1) and Cdc2 and activates p53-dependent apoptosis pathway in human breast cancer SK-BR-3 cells. J Nutr Biochem, 20: 285-290, 2009.
3. Hill, A. A., LaPan, P., Li, Y., and Haney, S. Impact of image segmentation on high-content screening data quality for SK-BR-3 cells. BMC Bioinformatics, 8: 340, 2007.
4. Koster, F., Felberbaum, R., Finas, D., Wunsch, K., Schulz, C., Diedrich, K., and Hauser, C. Progesterone and estradiol enhance lipid mediated transfection of Sk-Br-3 mammalian cancer cells. Int J Mol Med, 9: 617-620, 2002.
5. Lin, Y. J., Huang, Y. H., Zhen, Y. Z., Liu, X. J., and Zhen, Y. S. [Rhein lysinate induces apoptosis in breast cancer SK-Br-3 cells by inhibiting HER-2 signal pathway]. Yao Xue Xue Bao, 43: 1099-1105, 2008.
6. Manfroid, I., Van de Weerdt, C., Baudhuin, A., Martial, J. A., and Muller, M. EGF stimulates Pit-1 independent transcription of the human prolactin pituitary promoter in human breast cancer SK-BR-3 cells through its proximal AP-1 response element. Mol Cell Endocrinol, 229: 127-139, 2005.