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> Taq DNA Polymerase, 5u/ul

Taq DNA Polymerase, 5u/ul

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Cat# B0089-1,000U

Size : 1,000U

Brand : Bio Basic

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Taq DNA Polymerase, 5u/ul
Storage: (-15 to -20)C
Product Description: Taq DNA Polymerase, 5u/ul

Product information Taq DNA Polymerase (New Buffer!)

Product Description:
Taq DNA Polymerase is a thermosle DNA Polymerase isolated from a strain of Thermus sp. Taq has a half life of 3 hours at 95/10^6 (or 0.01/10^3) during DNA synthesis.

Taq is designed for use in primer extension reaction. Taq can also be used for sequencing. DNA sequencing at high temperature may decrease the second structure of some DNA templates and permit polymerization through baseug of Lambda/Hind III DNA is incubated with 20 units of the enzyme in assay buffer at 75 hrs and no visible contaminating activity is observed;

For exonucleases assay, 1ug of pBR322 plasmid DNA is incubated with 10 units of enzyme for 16 hrs at 75 units of Taq DNA Polymerase in 100ul of a reaction mixture for making first strand cDNA at beginning and no impaired effect on the first strand is observed.

Unit Definition:
One unit incorporates 10nmole of dNTP into acid min. at 74 units/ul in 100mM KCl, 20mM Tris HCl ( pH 8.0, 22mM EDTA, 0.5mM PMSF, 1mM DTT, 50% glycerol.

10 X Taq Reaction Buffer:
100mM KCl, 100mM (NH4)2SO4, 200mM Tris HCl (pH 8.75) at 22% Triton X and 1mg/ml BSA. Buffer is optimized for use with 200uM dNTPs.

Magnesium Sulfate:
20mM MgSO4. The final magnesium sulfate may be variable according to individual requirements. In general, 2mM MgSO4 is recommended. Primer Extension Characteristics: Taq has the independent terminal transferase activity which results in the addition of a single nucleotide (adenosine) at 3' end of the extension product. TA cloning vector is recommended if the extension product is needed to be cloned.
Total Product Size: B0089(D0089) (200U)
B0089(D0089) (500U)
B0089(D0089) (1000U)
B0089(D0089) (5000U)
Number of Containers: 1
Refrigeration Requirements: Freezer
Shipping Conditions: ICE
UNSPSC Code: 12352204
UNSPSC Category: DNA Polymerases
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Cat#
Description
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Price Bef. VAT
B0089-200U
Taq DNA Polymerase, 5u/ul
 200U