Total Glutathione (T-GSH)/Oxidized Glutathione (GSSG) Colorimetric Assay Kit

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Cat# E-BC-K097-M-96T

Size : 96T

Contact local distributor :

Phone : +1 850 650 7790

Brand : Elabscience

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Contact local distributor :

Phone : +1 850 650 7790

Detection Principle	GSSG is reduced to GSH by glutathione reductase, and GSH can react with DTNB to produce GSSG and yellow TNB. The amount of total glutathione (GSSG+GSH) determines the amount of yellow TNB. Thus the total glutathione can be calculated by measuring the OD value at 412 nm. The content of GSSG can be determined by first removing GSH from the sample with appropriate reagent and then using the above reaction principle.
Synonyms	GSSG;T-GSH
Sample Type	Serum,Plasma,Animal tissue,Red blood Cell,cultured Cell
Detection Method	Colorimetric method
Detection Instrument	Microplate reader (405-415 nm, optimum wavelength: 412 nm)
Research Area	Oxidative Stress , Cuproptosis , Ferroptosis
Other Reagents Required	PBS (0.01 mol/L, pH 7.4), Absolute ethanol
Storage	This product can be stored at -20°C for 12 months with shading light.
Valid Period	12 months
Sensitivity	0.36 μmol/L
Detection Range	0.36-30 μmol/L
Precision	inter-assay CV: 3.9% \| intra-assay CV: 0.6%
Assay Time	1 h 40 min

Cat.No.	Product Name	Sizes
E-BC-K030-M	Reduced Glutathione (GSH) Colorimetric Assay Kit	96T , 48T
E-BC-K096-M	Glutathione Peroxidase (GSH-Px) Activity Assay Kit	96T , 48T
E-BC-K099-S	Glutathione Reductase (GR) Activity Assay Kit	100Assays , 50Assays
E-BC-K278-S	Glutathione-S-Transferase (GST) Activity Assay Kit	100Assays , 50Assays

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type	Dilution Factor
Human plasma	1
10% Rat liver tissue homogenate	10-20
10% Rat kidney tissue homogenate	1
10% Rat heart tissue homogenate	10-20
10% Mouse brain tissue homogenate	2-5
HepG2 cells	1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.