TRK fused gene (TFG) (NM_006070) Human Over-expression Lysate
Cat# LC401827
Size : 20ug
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TRK fused gene (TFG) (NM_006070) Human Over-expression Lysate
SKU
LC401827
TFG HEK293T cell transient overexpression lysate (as WB positive control)
2 Weeks*
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone [RC201093] |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody ([TA50011-100]) |
| ACCN | NM_006070, NP_006061 |
| Synonyms | HMSNP; SPG57; TF6; TRKT3 |
| Predicted MW | 43.4 kDa |
| Components | 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate |
| Storage | The lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20°C. Lysate samples can be reconstituted with SDS Sample Buffer. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples are stable for 12 months from date of receipt when stored at -20°C. |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins. |
| Reference Data | |
| RefSeq | NP_006061 |
| Locus ID | 10342 |
| Cytogenetics | 3q12.2 |
| Protein Families | WB |
| Protein Pathways | Pathways in cancer, Thyroid cancer |
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Recombinant Protein Resources |
| SKU | Description | Size | ||
|---|---|---|---|---|
| PH300293 | TFG MS Standard C13 and N15-labeled recombinant protein (NP_001007566) | 10 ug | | |
| LC423514 | TFG HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug | | |
| LY401827 | Transient overexpression lysate of TRK-fused gene (TFG), transcript variant 1 | 100 ug | | |
| LY423514 | Transient overexpression lysate of TRK-fused gene (TFG), transcript variant 2 | 100 ug | | |
| TP300293 | Recombinant protein of human TRK-fused gene (TFG), transcript variant 2, 20 µg | 20 ug | | |
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.