Arl8b (NM_026011) Mouse Tagged ORF Clone
Referencia MR201737
embalaje : 10ug
Product Data | |
Target Symbol | Arl8b |
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Synonyms | 2610313E07Rik; 3100002J04Rik; Arl10c; gie1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data | ORF Nucleotide Sequence >MR201737 representing NM_026011 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGGCGCTCATCTCCCGCCTGCTGGACTGGTTCCGTTCGCTCTTCTGGAAGGAGGAGATGGAACTGA CGCTCGTGGGGCTGCAGTACTCCGGCAAGACCACCTTCGTCAATGTCATCGCGTCCGGTCAATTCAGTGA AGATATGATACCCACAGTGGGCTTCAACATGAGGAAAGTAACTAAAGGCAACGTCACAATAAAGATCTGG GACATAGGCGGACAGCCCCGGTTCCGGAGCATGTGGGAGCGGTACTGCCGAGGAGTCAATGCAATTGTTT ACATGATAGATGCTGCAGATCGAGAAAAGATAGAAGCCTCTCGAAATGAACTGCATAATCTTCTAGATAA ACCACAGTTACAAGGGATTCCAGTACTAGTACTTGGAAACAAGAGAGATCTTCCAAATGCCTTGGATGAG AAACAGCTAATTGAGAAAATGAACCTGTCTGCTATTCAGGATAGAGAAATTTGCTGCTATTCAATTTCTT GCAAAGAAAAGGATAATATAGATATCACACTTCAGTGGCTTATTCAACACTCAAAATCCCGGAGAAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence >MR201737 representing NM_026011 Red=Cloning site Green=Tags(s) MLALISRLLDWFRSLFWKEEMELTLVGLQYSGKTTFVNVIASGQFSEDMIPTVGFNMRKVTKGNVTIKIW DIGGQPRFRSMWERYCRGVNAIVYMIDAADREKIEASRNELHNLLDKPQLQGIPVLVLGNKRDLPNALDE KQLIEKMNLSAIQDREICCYSISCKEKDNIDITLQWLIQHSKSRRS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_026011 |
ORF Size | 558 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_026011.3, NP_080287.1 |
RefSeq Size | 2928 bp |
RefSeq ORF | 561 bp |
Locus ID | 67166 |
UniProt ID | Q9CQW2 |
Cytogenetics | 6 E2 |
MW | 22 kDa |
Summary | Plays a role in lysosome motility (PubMed:30174114). In neurons, mediates the anterograde axonal long-range transport of presynaptic lysosome-related vesicles required for presynaptic biogenesis and synaptic function (PubMed:30174114). May play a role in chromosome segregation (By similarity).[UniProtKB/Swiss-Prot Function] |
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Product Manuals |
FAQs |
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SDS |
SKU | Description | Size | ||
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MC200962 | Arl8b (untagged) - Mouse ADP-ribosylation factor-like 8B (Arl8b), (10ug) | 10 ug | | |
MG201737 | Arl8b (tGFP-tagged) - Mouse ADP-ribosylation factor-like 8B (Arl8b) | 10 ug | | |
MR201737L3 | Lenti ORF clone of Arl8b (Myc-DDK-tagged) - Mouse ADP-ribosylation factor-like 8B (Arl8b) | 10 ug | | |
MR201737L4 | Lenti ORF clone of Arl8b (mGFP-tagged) - Mouse ADP-ribosylation factor-like 8B (Arl8b) | 10 ug | | |
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.