Arl8a (NM_026823) Mouse Tagged ORF Clone
Referencia MR201740
embalaje : 10ug
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Arl8a (NM_026823) Mouse Tagged ORF Clone
SKU
MR201740
Arl8a (Myc-DDK-tagged) - Mouse ADP-ribosylation factor-like 8A (Arl8a)
- TrueORF® TrueORF®
Expression-ready ORF plasmid with C-terminal tag(s)
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| Product Data | |
| Target Symbol | Arl8a |
|---|---|
| Synonyms | 1110033P22Rik; Arl10b; gie2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | ORF Nucleotide Sequence >MR201740 representing NM_026823 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATCGCTTTGTTCAACAAGCTGCTGGACTGGTTCAAGGCCCTGTTCTGGAAGGAGGAAATGGAGCTCA CGCTGGTGGGGCTGCAGTACTCGGGCAAGACCACCTTCGTCAACGTGATCGCGTCAGGACAGTTCAATGA GGACATGATCCCCACTGTGGGTTTCAACATGCGAAAAATCACCAAAGGGAATGTGACCATCAAGCTCTGG GACATTGGGGGGCAGCCCCGTTTCCGAAGCATGTGGGAACGCTACTGCAGAGGAGTGAGCGCCATTGTGT ATATGGTGGATGCTGCGGATCAGGAGAAGATCGAGGCCTCCAAGAATGAGCTCCACAACCTGCTAGACAA GCCACAGCTACAAGGCATCCCGGTCTTAGTCCTTGGTAACAAGCGAGACCTCGCCGGAGCGCTAGATGAG AAGGAGCTAATCGAGAAAATGAACCTGTCTGCTATCCAGGACCGAGAGATCTGCTGCTACTCCATATCCT GCAAAGAGAAGGACAACATAGACATCACCCTACAGTGGCTTATTCAACACTCAAAGTCACGGAGAAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence >MR201740 representing NM_026823 Red=Cloning site Green=Tags(s) MIALFNKLLDWFKALFWKEEMELTLVGLQYSGKTTFVNVIASGQFNEDMIPTVGFNMRKITKGNVTIKLW DIGGQPRFRSMWERYCRGVSAIVYMVDAADQEKIEASKNELHNLLDKPQLQGIPVLVLGNKRDLAGALDE KELIEKMNLSAIQDREICCYSISCKEKDNIDITLQWLIQHSKSRRS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms | Chromatograms Sequencher program is needed, download here |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
| ACCN | NM_026823 |
| ORF Size | 558 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_026823.2, NP_081099.1 |
| RefSeq Size | 1702 bp |
| RefSeq ORF | 561 bp |
| Locus ID | 68724 |
| UniProt ID | Q8VEH3 |
| Cytogenetics | 1 E4 |
| MW | 21.8 kDa |
| Summary | Plays a role in lysosomes motility (PubMed:30174114). In neurons, mediates the anterograde axonal long-range transport of presynaptic lysosome-related vesicles required for presynaptic biogenesis and synaptic function (PubMed:30174114). May play a role in chromosome segregation (By similarity).[UniProtKB/Swiss-Prot Function] |
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| Product Manuals |
| FAQs |
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| SDS |
| SKU | Description | Size | ||
|---|---|---|---|---|
| MC201644 | Arl8a (untagged) - Mouse ADP-ribosylation factor-like 8A (Arl8a), (10ug) | 10 ug | | |
| MG201740 | Arl8a (tGFP-tagged) - Mouse ADP-ribosylation factor-like 8A (Arl8a) | 10 ug | | |
| MR201740L3 | Lenti ORF clone of Arl8a (Myc-DDK-tagged) - Mouse ADP-ribosylation factor-like 8A (Arl8a) | 10 ug | | |
| MR201740L4 | Lenti ORF clone of Arl8a (mGFP-tagged) - Mouse ADP-ribosylation factor-like 8A (Arl8a) | 10 ug | | |
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.