K2120-100 assays | Adipogenesis Colorimetric/Fluorometric Assay Kit

Background

Adipogenesis is the process of preadipocytes differentiate into adipocytes, the primary fat storage cells. Adipocytes play a critical role in energy homeostasis and store the energy as triglycerol in animals. Adipocytes accumulate triglycerides in the form of lipid droplets that can be measured. The accumulation of adipocytes leads to obesity, a main risk factor in many diseases, including cardiovascular disease, atherosclerosis, diabetes and cancer, etc.

The Adipogenesis Colorimetric/Fluorometric Assay Kit provides a highly sensitive, fast and convenient way for detection of triglyceride accumulation in cells and tissues based on colorimetric and fluorometric method. In the assay, triglycerides are efficiently solubilized and then hydrolyzed to glycerols which are subsequently oxidized to convert the probe to yield fluorescence (Ex/Em = 535/587 nm) and color (λmax = 570 nm). The kit with a convenient microplate assay format is a convenient tool for studying the effect of adipogenesis inhibitors or inducers, or to screen drugs. The kit can quantify triglyceride in as few as a thousand or less differentiated 3T3-L1 cells with detection linear range 0.2 to 10 nmol.

Features & Properties

Features	Fast and convenient; Simple procedure; Takes approx. 40 minutes; Kit contains all necessary reagents for rapid, sensitive and accurate measurement of adipogenesis in various samples.
Shipping	Blue Ice
Storage Conditions	Store at -20°C.

Quality Control

DataSheet

Storage

Store at -20°C.

Overview

Kit components

Adipogenesis Assay Buffer, Lipid Extraction Solution, Adipogenesis Probe (in DMSO solution), Lipase (lyophilized), Adipogenesis Enzyme Mix (lyophilized), Triglyceride Standard (1 mM)

Adipogenesis Colorimetric/Fluorometric Assay Kit

Referencia K2120-100 assays

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