Angiopoietin 2 antibody [Angy-2-1]
Referencia GTX33916-100ug
embalaje : 100ug
Marca : GeneTex
Contact local distributor :
Teléfono : +1 850 650 7790
![Anti-Angiopoietin 2 antibody [Angy-2-1] used in (). GTX33916](http://www.genetex.com/templates/default/js/lightbox/images/loading.gif "Anti-Angiopoietin 2 antibody [Angy-2-1] used in (). GTX33916")
Host | Mouse |
|---|---|
Clonality | Monoclonal |
Clone Name | Angy-2-1 |
Isotype | IgG2b |
Application | ELISA, Neutralizing/Inhibition |
Reactivity | Human, Mouse |
| Package | 100 μg |
Datasheet File View product citations for antibody GTX33916 on CiteAb
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application | Recommended Dilution |
|---|---|
| ELISA | Assay dependent |
| Neutralizing/Inhibition | Assay dependent |
| Note : Neutralizing/InhibitionInhibits the binding of mouse angiopoietin-2 to mouse Tie-2. ND50* = 50-60ng/ml (for 10ng/ml of mouse Angiopoietin-2) Inhibits the binding of human angiopoietin-2 to human Tie-2. ND50* = 8-12ng/ml (for 10ng/ml of human Angiopoietin-2) *ND50: 50% neutralizing dose of antibody for a given concentration of ligand (here Angiopoietin-2). | |
Not tested in other applications.
Calculated MW
57 kDa. ( Note )
Product Note
Recognizes human and mouse angiopoietin-2. Does not detect human angiopoietin-1.
PROPERTIES
Form
Liquid
Buffer
PBS, 10% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant human angiopoietin-2.
Purification
Protein A-affinity purified.
Purity
??95% by SDS-PAGE.
Conjugation
Unconjugated
RRID
AB_2887728
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
angiopoietin 2 , AGPT2 , ANG2
Cellular Localization
Secreted
Background
The protein encoded by this gene is an antagonist of angiopoietin 1 (ANGPT1) and endothelial TEK tyrosine kinase (TIE-2, TEK). The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Database
DATA IMAGES
| | GTX33916 Image
|
| | GTX33916 ELISA ImageBinding of human Angiopoietin-2 to Tie-2 (human):Fc is inhibited by the antibody anti-Angiopoietin-2, mAb (rec.) (blocking) (Angy-2-1). Methods: Tie-2 (h):Fc was coated on an ELISA plate at 1μg/ml. anti-Angiopoietin-2, mAb (rec.) (blocking) (Angy-2-1) or an unrelated mAb (recombinant) (Control) were added (starting at 40μg/ml with a twofold serial dilution) together with 20ng/μl of Angiopoietin-2 (human). After incubation for 1h at RT, the binding was detected using an anti-FLAG antibody (HRP). |
Application Reference
Zhong J et al. Phytomedicine 2020; 78 : 153302 Notoginsenoside R1 activates the Ang2/Tie2 pathway to promote angiogenesis.
Application : Neutralizing/Inhibition
Clone Reference
An YA et al. Elife 2017; 6. pii:e24071 Angiopoietin-2 in white adipose tissue improves metabolic homeostasis through enhanced angiogenesis.
Application : Neutralizing/Inhibition
Reactivity : Mouse
Yun JH et al. Cell Death Dis. 2016; 7:e2101 Angiopoietin 2 induces astrocyte apoptosis via αvβ5-integrin signaling in diabetic retinopathy.
Application : Neutralizing/Inhibition
Reactivity : Mouse
REVIEW
Application Reference
Zhong J et al. Phytomedicine 2020; 78:153302 Notoginsenoside R1 activates the Ang2/Tie2 pathway to promote angiogenesis.
Application : Neutralizing/Inhibition
Clone Reference
An YA et al. Elife 2017; 6. pii:e24071 Angiopoietin-2 in white adipose tissue improves metabolic homeostasis through enhanced angiogenesis.
Application : Neutralizing/Inhibition
Reactivity : Mouse
Yun JH et al. Cell Death Dis. 2016; 7:e2101 Angiopoietin 2 induces astrocyte apoptosis via αvβ5-integrin signaling in diabetic retinopathy.
Application : Neutralizing/Inhibition
Reactivity : Mouse
| SDS | |
|---|---|
| PBS.pdf | |
| Glycerol.pdf | |
| Sodium Azide.pdf | |