Anti-ALPHA-1-ANTI-TRYPSIN (RABBIT) Antibody

Referencia 600-401-147-0100

embalaje : 100ug

Marca : Rockland Immunochemicals

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Specifications for Alpha-1-Anti-Trypsin Antibody

Product Details

Anti-Alpha-1-Anti-Trypsin (RABBIT) Antibody - 600-401-147
rabbit anti-alpha-1-antitrypsin antibody, A1A antibody, A1AT, AAT, Alpha 1 antiproteinase antibody, Alpha 1 antitrypsin, Alpha 1 protease inhibitor, alpha-1-AT antibody, alpha1 PI antibody, alpha1 proteinase inhibitor antibody, Serpin A1, Anti-α-1-antitrypsin
Rabbit
Polyclonal
IgG

Target Details

SERPINA1  - View All SERPINA1 Products
Human
Native Protein
Human Alpha-1-anti-Trypsin
Anti-Human ALPHA-1-ANTI-TRYPSIN Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigen resins attached to a solid phase.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.  Analysis by SDS-PAGE was used to determine that this preparation is substantially free of aggregates and shows a banding pattern consistent with purified Rabbit IgG.
P01009 - UniProtKB
NP_000286.3 - NCBI Protein
5265 - Gene ID

Application Details

ELISA, WB
Anti-Human ALPHA-1-ANTI-TRYPSIN Antibody has been tested by ELISA, and western blot. This product is assayed against 1.0 µg of Human Alpha-1-anti-Trypsin in a standard ELISA using Peroxidase conjugated Affinity Purified Donkey anti-Rabbit IgG and TMB as a substrate for 30 minutes at room temperature.  A working dilution of 1:10,000 to 1:50,000 is suggested for this product.

Formulation

1.3 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None

Shipping & Handling

Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.

Background

ALPHA-1-ANTI-TRYPSIN Antibody is specific for alpha-1-anti-trypsin. Alpha 1-Antitrypsin or α1-antitrypsin (A1AT) is a protease inhibitor belonging to the serpin superfamily and is generally known as serum trypsin inhibitor. Alpha-1-anti-trypsin protects tissues from enzymes of inflammatory cells. A1AT antibody is suitable for immunology and cardiovascular researcher.

Related Protocols

Adherent Cell Lysis Protocol
ELISA Protocol
Fluorescent Western Blotting Protocol
Histone Immunoblotting Protocol
In-Cell Western (ICW) Protocol
IP-WB with TrueBlot® Protocol
Multi-Lysate Western Blotting Protocol
Nuclear & Cytoplasmic Extract Protocol
Sandwich ELISA Protocol for Collagen
Suspension Cultured Cell Lysis Protocol
Western Blotting (WB) Protocol

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Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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