Anti-BOVINE SERUM (RABBIT) Antibody Peroxidase Conjugated

Referencia 201-4301-0100

embalaje : 100ug

Marca : Rockland Immunochemicals

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Specifications for Bovine Serum Antibody Peroxidase Conjugated

Product Details

Anti-Bovine Serum (RABBIT) Antibody Peroxidase Conjugated (BULK ORDER) - 201-4301
Rabbit anti-Bovine Serum Peroxidase Conjugated Antibody, Rabbit anti-Bovine Serum Antibody HRP Conjugation
Rabbit
Peroxidase (HRP)
Polyclonal
IgG

Target Details

Bovine
Native Protein
Bovine serum proteins
Bovine serum is an IgG fraction antibody purified from polyspecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and anti-Peroxidase.  Multiple precipitin arcs were observed against Bovine Serum.

Application Details

Dot Blot
WB  - View References
Anti-Bovine Serum peroxidase conjugate has been tested by dot blot and is suitable to be assayed against 1.0 ug of Bovine IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product.

Formulation

10.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
10 mg/ml Polyethylene Glycol (PEG-8000)
2.0 mL
Restore with deionized water (or equivalent)

Shipping & Handling

Ambient
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.

Background

Anti-Bovine serum antibody detects bovine serum proteins. Serum proteins are those proteins remaining in portion of plasma after coagulation of blood, during which process the plasma protein fibrinogen is converted to fibrin and remains behind in the clot. Anti-Bovine serum antibody is ideal for investigators involved in Cell Signaling, Cellular Biology and Signal Transduction research.

References (1)

(). The pro-adhesive and pro-survival effects of glucocorticoid in human ovarian cancer cells. J Mol Endocrinol.
Applications
WB, IB, PCA
PubMed

Related Protocols

Adherent Cell Lysis Protocol
Fluorescent Western Blotting Protocol
General Immunoblotting Protocol
Histone Immunoblotting Protocol
In-Cell Western (ICW) Protocol
IP-WB with TrueBlot® Protocol
Multi-Lysate Western Blotting Protocol
Nuclear & Cytoplasmic Extract Protocol
Suspension Cultured Cell Lysis Protocol
Western Blotting (WB) Protocol

Certificate of Analysis Lookup

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Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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