ELISA shows that clone RM129 does not react to monkey IgG. The plate was coated with Rhesus monkey IgG. A serial dilution of RM129 and a monkey IgG reacting antibody (positive control) was used as the detection antibodies.

ELISA shows that clone RM129 reacts only to the kappa and lambda light chains in all human immunoglobulins, and does not react to mouse IgG, rat IgG, or goat IgG.

A titer ELISA using RM129. The plate was coated with different amounts of human IgG4k. A serial dilution of RM129 was used as the primary antibody, followed by an alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

A titer ELISA using RM129. The plate was coated with different amounts of human IgG3λ. A serial dilution of RM129 was used as the primary antibody, followed by an alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.