| Description | Anti-MOUSE IgM (H&L) (RABBIT) Antibody Peroxidase Conjugated |
|---|---|
| Host | Rabbit |
| Conjugate | Peroxidase (Horseradish) |
| Target Species | Mouse |
| Reactivity | Mouse |
| Clonality | Polyclonal |
Application
| WB, E, IC |
| Application Note | ELISA 1:10,000-1:50,000 Western Blot 1:1,000-1:10,000 Immunochemistry 1:500-1:2,500 |
| Physical State | Lyophilized |
| Host Isotype | IgG |
| Target Isotype | IgM (H&L) |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Immunogen | Anti-Mouse IgM (H&L) was produced by repeated immunization with mouse IgM whole molecule in rabbit. |
| Reconstitution Volume | 2.0 mL |
| Reconstitution Buffer | Restore with deionized water (or equivalent) |
| Preservative | 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! |
| Shipping Condition | Ambient |
|---|---|
| Purity | This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Rabbit Serum. This reagent primarily recognizes Mouse IgM however some light chain cross reactivity will occur against other mouse immunoglobulins. |
| Storage Condition | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
Research Areas
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Application Protocols
Provided below are standard protocols that you may find useful for product applications.
Background
Anti-Mouse IgM (H&L) peroxidase conjugated antibody generated in rabbit detects specifically mouse IgM (H&L). This secondary peroxidase conjugated antibody anti-Mouse is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Availability: 2-3 weeks
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Cat# ASR2928