Anti-TNNT2 (Cardiac Muscle Troponin T, Troponin T, Cardiac Muscle, Troponin T type 2 (cardiac), cTnT, TnTC) Polyclonal Antibody

Troponin, working in conjunction with tropomyosin, functions as a molecular switch, regulating muscle contraction in response to changes in the intracellular Ca2+ concentration. Troponin consists of three subunits: the Ca2+-binding subunit troponin C (TnC), the tropomyosin-binding subunit troponin T (TnT), and the inhibitory subunit troponin I (TnI) (1). In response to beta-adrenergic stimulation of the heart, Ser23 and Ser24 of TnI (cardiac) are phosphorylated by PKA and PKC. This phosphorylation stimulates a conformational change of the regulatory domain of TnC, reduces the association between TnI and TnC, and decreases myofilament Ca2+ sensitivity by reducing the Ca2+ binding affinity of TnC (1-3).||The tropomyosin binding subunit of the troponin complex TnT exists as different isoforms in slow skeletal muscle (ssTnT/TNNT1), fast skeletal muscle (fsTnT/TNNT3) and in cardiac muscle (cTnT/TNNT2). Each of these may also contain multiple alternatively spliced variants. Assays for measuring serum concentrations of cTnT, as well as cTnI, have been reported for analyzing cardiac injury.||Applications: |Suitable for use in Western Blot. Other applications not tested.||Recommended Dilution:|Western Blot: 1:500-1:2000|Optimal dilutions to be determined by the researcher.||Storage and Stability: |May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.