Aspartate Aminotransferase (AST/GOT) Activity Assay Kit
Referencia E-BC-K236-M-48T
embalaje : 48T
Marca : Elabscience
Contact local distributor :
Teléfono : +1 850 650 7790
| Detection Principle | AST/GOT enables alpha-ketoglutaric acid and aspartic acid to displace amino and keto groups to form glutamic acid and oxaloacetic acid. Oxaloacetic acid can decarboxylate itself to form Pyroracemic acid during the reaction. Pyroracemic acid reacted with 2,4-dinitrophenylhydrazine(DNPH) to form 2,4, dinitrophenylhydrazone showing reddish brown in alkaline solution. Measure the OD values and calculate the enzyme activity. |
| Synonyms | AST;GOT;Glutamic-oxalacetic transaminase |
| Sample Type | Serum,Plasma,Animal tissue,Cell,Cell culture supernatant |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (500-520 nm) |
| Research Area | Liver And Renal Function |
| Other Reagents Required | Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4) |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 1.10 IU/L |
| Detection Range | 1.10-72.30 IU/L |
| Precision | inter-assay CV: 6.8% | intra-assay CV: 5.3% |
| Assay Time | 1 h 20 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 1 |
| Human plasma | 1 |
| Porcine serum | 1 |
| Rat serum | 1 |
| HC-60 cellular supernatant | 1 |
| Calu-3 cellular supernatant | 1 |
| 10% Rat liver tissue homogenization | 15-30 |
| 10% Rat lung tissue homogenization | 2-8 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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