Differentiation of Group D from non-Group D Streptococci.||Bile Esculin Agar is prepared according to the formula specified by Swan, and later evaluated by Facklam and Moody. It was concluded that the bile esculin test provided a reliable means of identifying enterococci and differentiation from non-enterococci. Enterococci are bile tolerant and hydrolyze esculin which imparts a dark brown color to the medium. Gram positive bacteria other than enterococci are inhibited by the bile salts.||Appearance: |Light beige, homogeneous and free flowing||Solubility: |Grayish yellow, opalescent, trace to light haze, none to light precipitate||pH: |6.6 ± 0.2 ||Microbiological Analysis:|Enterococcus faecalis (ATCC19433); Good Black colonies and medium|Enterococcus faecalis (ATCC 29212); Good Black colonies and medium|Enterococcus faecalis (ATCC33186); Good Black colonies and medium|Escherichia coli (ATCC 25922); Good Colorless No blackening|Streptococcus pyogenes (ATCC 19615); Inhibited N/A||*The results were obtained from type cultures after incubation at a temperature of 35ºC and observed after 18-24 hours. ||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.