Bovine serum albumin (also known as BSA or "Fraction V") is a globular protein used extensively as a carrier protein to dilute antibodies. Because is a small, stable, moderately non-reactive protein, it is often used as a general protein blocking agent in immunoassays and immunodetection protocols. BSA immunoassay applications include ELISA (Enzyme-Linked Immunosorbent Assay), immunoblots (western blot and dot blot), and immunocytochemistry (immunohistochemistry and immunofluorescence microscopy). Bovine BSA Standard Powder Protein is a purified protein or bioactive peptide.
Research Articles on Bovine Serum Albumin (BSA)
| Target | Bovine Serum Albumin (BSA) |
| Origin | Cow |
| Tested Applications | ELISA, WB, IHC |
| Host | Cow |
| Recommended dilutions | Suitable for us in ELISA, WB and IHC-F, not tested in IHC-P. Optimal dilutions/concentrations should be determined by the end user. |
| Conjugation | Unconjugated |
| Form | Lyophilized |
| Purity | > 96% Albumin: 99% (Agarose Electrophoresis) Protein 99% (Biuret) Moisture: < 2.0% Ash: < 2.0% Heavy Metals: < 0.1 ppm (Lead) Tested negative for Blue Tongue Virus and Vesicular Stomatitis Virus. |
| Purification | Purified by a proprietary heat-shock process. |
| Storage | Store lyophilized between 2-8 °C. After reconstitution, aliquot and store between -15 and -25 °C. Avoid repeated freeze/thaw cycles. |
| Buffer | pH: 7.0. Does not contain preservatives. |
| Availability | Shipped within 10-20 working days. |
| Note | This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use. |
| Directions for use | Centrifuge before opening to ensure complete recovery of vial contents. |