Capan-1 cells
Referencia C0018001
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Disease:
Pancreatic adenocarcinoma Established from liver Homo sapiens Pancreas Epithelial Adherent fibroblastic semi-confluent monolayer forming loosely adherent foci Tumorigenic; hypotriploid with abnormalities Male, Caucasian, 40 yrs of age IMDM + 20% FBS 1:2 to 1:4 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37°C Complete culture medium supplemented with 5% (v/v) DMSO Bacteria: Negative Yeast: Negative Mycoplasma: Negative HIV: Negative Hepatitis B: Negative Hepatitis C: Negative AR-42J cells BxPc-3 cells Capan-1 cells MIA PaCa-2 cells E3LZ10.7 cells A10.7 cells INS-1 cells MIN-6 cells Origin:
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Biosafety Level:
I Sterility:
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Protein Expression:
mucin, progesterone receptor, estrogen receptor alpha, HLA A2, A9, B13, B17 Isoenzymes:
AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 1-2; Me-2, 1; PGM1, 1-2; PGM3, 1; DNA Profile (STR):
Amelogenin: X; CSF1PO: 11; D13S317: 9; D16S539: 13,14; D5S818: 11; D7S820: 10,11; THO1: 6; TPOX: 8,11; vWA: 16 Related cells by Organ:
References for Capan-1 cells:
1. Holt, J. T., Toole, W. P., Patel, V. R., Hwang, H., and Brown, E. T. Restoration of CAPAN-1 cells with functional BRCA2 provides insight into the DNA repair activity of individuals who are heterozygous for BRCA2 mutations. Cancer Genet Cytogenet, 186: 85-94, 2008.
2. Fanjul, M., Alvarez, L., and Hollande, E. Expression and subcellular localization of a 35-kDa carbonic anhydrase IV in a human pancreatic ductal cell line (Capan-1). J Histochem Cytochem, 55: 783-794, 2007.
3. Szucs, A., Demeter, I., Burghardt, B., Ovari, G., Case, R. M., Steward, M. C., and Varga, G. Vectorial bicarbonate transport by Capan-1 cells: a model for human pancreatic ductal secretion. Cell Physiol Biochem, 18: 253-264, 2006.
4. Liu, Z. W., Zhao, Y. P., Liao, Q., Wu, Y. D., and Zhu, Y. [Relationship between apoptosis induced by 2-butylamino-2-demethoxy-hypocrellin B in human pancreatic cancer cells Capan-1 and photosensitization of mitochondria]. Zhonghua Wai Ke Za Zhi, 44: 36-39, 2006.
5. Gay, M., Montana, A. M., Moreno, V., Prieto, M. J., Llorens, R., and Ferrer, L. Studies of interaction of dichloro[eta2-dimethyl-(2-methylidene-cyclohexylmethyl)-amino]platinum(II) with DNA: effects on secondary and tertiary structures of DNA - cytotoxic assays on human cancer cell lines Capan 1 and A431. J Inorg Biochem, 99: 2387-2394, 2005.
6. McCabe, N., Lord, C. J., Tutt, A. N., Martin, N. M., Smith, G. C., and Ashworth, A. BRCA2-deficient CAPAN-1 cells are extremely sensitive to the inhibition of Poly (ADP-Ribose) polymerase: an issue of potency. Cancer Biol Ther, 4: 934-936, 2005.
7. Shirk, A. J. and Kuver, R. Epidermal growth factor mediates detachment from and invasion through collagen I and Matrigel in Capan-1 pancreatic cancer cells. BMC Gastroenterol, 5: 12, 2005.