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Reactivos e instrumentos para inmunología, biología celular y biología molecular.

Catalase (CAT) Activity Assay Kit

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Referencia E-BC-K031-M-96T

embalaje : 96T

Contact local distributor :

Teléfono : +1 850 650 7790

Marca : Elabscience

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Contact local distributor :

Teléfono : +1 850 650 7790

Detection Principle	The reaction that catalase (CAT) decomposes H₂O₂ can be quickly stopped by ammonium molybdate. The residual H₂O₂ reacts with ammonium molybdate to generate a yellowish complex. CAT activity can be calculated by production of the yellowish complex at 405 nm.
Synonyms	CAT
Sample Type	Serum,Plasma,Cell,Cell culture supernatant,Tissue
Detection Method	Colorimetric method
Detection Instrument	Microplate reader (400-410 nm, optimum wavelength: 405 nm)
Research Area	Oxidative Stress
Other Reagents Required	Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4)
Storage	This product can be stored at 2-8°C for 12 months with shading light.
Valid Period	12 months
Sensitivity	1.12 U/mL
Detection Range	1.12-150 U/mL
Precision	inter-assay CV: 7.7% \| intra-assay CV: 3.9%
Assay Time	40 min

Cat.No.	Product Name	Sizes
E-BC-K020-M	Total Superoxide Dismutase (T-SOD) Activity Assay Kit (WST-1 Method)	500Assays , 96T , 48T
E-BC-K025-M	Malondialdehyde (MDA) Colorimetric Assay Kit (TBA Method)	500Assays , 96T , 48T

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type	Dilution Factor
Human serum	1
293T supernatant	1
10% Rat heart tissue homogenization	50-100
10% Rat liver tissue homogenization	100-200
10% Rat spleen tissue homogenization	50-100
Mouse serum	1
10% Epipremnum aureum tissue homogenization	1-2
10% Rat lung tissue homogenization	50-100
10% Rat kidney tissue homogenization	50-100
10% Rat brain tissue homogenization	20-50

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.