Catalase (CAT) Activity Assay Kit
Referencia E-BC-K031-S-100
embalaje : 100Assays
Marca : Elabscience
Detection Principle | The reaction that catalase (CAT) decomposes H2O2 can be quickly stopped by ammonium molybdate. The residual H2O2 reacts with ammonium molybdate to generate a yellowish complex. CAT activity can be calculated by production of the yellowish complex at 405 nm. |
Synonyms | CAT |
Sample Type | Serum,Plasma,Cell,Tissue |
Detection Method | Colorimetric method |
Detection Instrument | Spectrophotometer (405 nm) |
Research Area | Oxidative Stress |
Other Reagents Required | Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4) |
Storage | This product can be stored at 2-8°C for 12 months with shading light. |
Valid Period | 12 months |
Sensitivity | 0.27 U/mL |
Detection Range | 0.27-155.4 U/mL |
Precision | inter-assay CV: 5.1% | intra-assay CV: 3.1% |
Assay Time | 1 h 10 min |
The recommended dilution factor for different samples is as follows (for reference only):
Sample Type | Dilution Factor |
---|---|
10% Rat liver tissue homogenization | 25-50 |
10% Rat kidney tissue homogenization | 10-25 |
10% Rat brain tissue homogenization | 5-10 |
Human serum | 1 |
293T supernatant | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
Other Clones
{{antibodyDetailsPage.numTotal}} Results
- {{item.title}}
Citations ({{item.publications_count}}) Manual MSDS
Cat.No.:{{item.cat}}
{{index}} {{goods_show_value}}
Other Formats
{{formatDetailsPage.numTotal}} Results
- {{item.title}}
Citations ({{item.publications_count}}) Manual MSDS
Cat.No.:{{item.cat}}
{{index}} {{goods_show_value}}
-
IF:{{item.impact}}
Journal:{{item.journal}} ({{item.year}})
DOI:{{item.doi}}Reactivity:{{item.species}}
Sample Type:{{item.organization}}
-
Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}}