CF®594 TUNEL Assay Apoptosis Detection Kit
Referencia 30064
embalaje : 1kit
Marca : Biotium
Contact local distributor :
Teléfono : +1 850 650 7790
| Apoptosis/viability marker | DNA fragmentation |
|---|---|
| For live or fixed cells | For fixed cells/tissue sections |
| Detection method/readout | Fluorescence microscopy, Flow cytometry |
| Assay type/options | Endpoint assay |
| Fixation options | Fix before staining (formaldehyde), Fix before staining (methanol), Permeabilize before staining |
| Colors | Green, Red, Far-red |
| Product origin | TdT enzyme (calf thymus); recombinant, produced in E. coli |
| Storage Conditions | Store at -10 to -35 °C, Protect from light |
Product Description
These kits use enzymatic labeling of DNA double-strand breaks for fluorescence staining of apoptotic cells in fixed samples or tissue sections by TUNEL.
Features
- Direct labeling of DNA ds breaks with fluorescent dUTP
- Bright & photostable next-generation CF® dyes
- For fixed cells or tissue sections
- Detect by fluorescence microscopy or flow cytometry
Kit Components
- Equilibration Buffer
- CF® Dye TUNEL Reaction Buffer
- TdT Enzyme (calf thymus; recombinant, produced in E. coli)
Note: Kits include reagents for 50 reactions using 50 uL per reaction.
In TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling), TdT enzyme catalyzes the addition of labeled dUTP to the 3′- ends of the internucleosomal DNA strand breaks that are a hallmark of apoptosis. The CF® dye TUNEL Assay Apoptosis Detection Kits employs dUTP conjugated to the exceptionally bright and photostable fluorescent dyes, for single step fluorescent TUNEL labeling of fixed cells or tissues for analysis by fluorescence microscopy or flow cytometry. Learn more about Biotium’s next-generation CF® Dyes.
See our full selection of Cell Viability & Apoptosis Kits.
CF® Dye TUNEL Assay Apoptosis Detection Kits
| Dye | Ex/Em | Size | Catalog No. |
|---|---|---|---|
| CF®488A | 490/515 nm | 50 reactions | 30063 |
| CF®594 | 593/614 nm | 50 reactions | 30064 |
| CF®640R | 642/662 nm | 50 reactions | 30074 |
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