Citrate BioAssay™ Kit

Citrate is an intermediate in the citric acid cycle and is involved in fatty acid synthesis. The Citrate Assay Kit provides a simple, and automation-ready procedure for measuring citrate concentration. Citrate is converted into pyruvate which is then oxidized with the conversion of the dye into a colored and fluorescent form. The color intensity at 570nm or fluorescence intensity at λex/em=530/585nm is directly proportional to the citrate concentration in the sample.||Intended Use:|For citrate determination in biological samples (e.g. serum, plasma, urine, tissue and culture media).||Key Features:|Fast and sensitive: Linear detection range: 4 to 400uM citrate for|colorimetric assays and 0.5 to 40uM for fluorimetric assays.||Convenient and high-throughput: Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling|systems for processing thousands of samples per day.||Kit Components:|172366A: Developer, 1x10ml|172366B: Dye Reagent, 1x120ul|172366C: Citrate Standard, 1x500ul|172366D: CL Enzyme, 1x1vial|172366E: ODC Enzyme, 1x120ul ||Storage and Stability:|Store all components at -20°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.