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> Cytokeratin 8/18(C-51), APC conjugate, 0.1mg/mL

Cytokeratin 8/18(C-51), APC conjugate, 0.1mg/mL

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Referencia BNCA0034-250

embalaje : 250uL

Marca : Biotium

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Product Attributes

Antibody number

#0034
Antibody reactivity (target)

Cytokeratin 8/18
Antibody type

Primary
Host species

Mouse
Clonality

Monoclonal
Clone

C-51
Isotype

IgG1, kappa
Molecular weight

52.5 kDa (CK8); 45 kDa (CK18)
Synonyms

CARD2; CK8; CYK8; CYKER; Cytokeratin Endo A; DreK8; EndoA; K2C8; K8; Keratin 8; Krt 2.8; KRT8; Type-II Keratin Kb8
Human gene symbol

KRT8 & KRT18
Entrez gene ID

3856 (CK8); 3875 (CK18)
SwissProt

P05787 (CK8); P05783 (CK18)
Unigene

533782 & 708445 (CK8); 406013 (CK18)
Immunogen

Cytoskeleton preparation from HeLa cells
Antibody target cellular localization

Cytoskeleton
Verified antibody applications

IHC (FFPE) (verified)
Species reactivity

Cow, Human, Monkey, Pig, Sheep
Expected antibody applications

IF (published for clone), WB (published for clone)
Antibody application notes

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody, Immunofluorescence: 1-2 ug/mL, Does not react with mouse, rat, ferret, rabbit, donkey, chicken, Xenopus; others not known, Immunohistology formalin-fixed 0.5-1.0 ug/mL, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes, Flow Cytometry 0.5-1 ug/million cells/0.1 mL, Western blotting 0.5-1.0 ug/mL, Optimal dilution for a specific application should be determined by user
Positive control

MCF-7 or A431 cells. Skin, Colon, lung or breast carcinoma
Shipping condition

Room temperature
Storage Conditions

Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C
Shelf life

Guaranteed for at least 24 months from date of receipt when stored as recommended
Regulatory status

For research use only (RUO)
Antibody/conjugate formulation

Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide
Antibody research areas

Cancer, Cytoskeleton
Product origin

Product may contain either bovine serum albumin (BSA) from bovine serum (Bos taurus), or recombinant BSA produced in Chinese hamster ovary cells. Inquire for the specific lot.
Cell/tissue expression

Epithelial cells
Tumor expression

Breast cancer, Hepatocellular carcinoma, Paget cell marker, Sarcoma

Technology

CF® Dyes
Considerations for Immunofluorescence Staining
Primary & Secondary Antibody Conjugates
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