Cytokeratin, Basal Cell (APC)

Referencia C9097-03B-APC-100ul

embalaje : 100ul

Marca : US Biological

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C9097-03B-APC Cytokeratin, Basal Cell (APC)

Clone Type
Polyclonal
Host
mouse
Source
human
Swiss Prot
P13647
Isotype
IgG1
Grade
Affinity Purified
Applications
IHC WB
Crossreactivity
Ca Ch Hm Hu Po Rb Rt
Accession #
NM_000424.3
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze

Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight from between 40kD and 68kD and isoelectric pH between 4.9-7.8. The individual cytokeratin polypeptides are designated 1-20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies.||Applications:|Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.||Recommended Dilution:|Western Blot: 1:100-1:1000|Immunohistochemistry (frozen tissue sections): 1:100-1:200|Optimal dilutions to be determined by the researcher.||Storage and Stability:|Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.||Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG1|Clone No: 2Q1113|Host: mouse|Source: human|Concentration: As Reported |Form: Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).|Purity: Purified by Protein G affinity chromatography.|Immunogen: Human cytokeratins. |Specificity: Recognizes human cytokeratin 5. Stains basal cells in combined and stratified epithelial tissues. Recognizes the stem cell population, including the amplifying cell in the prostate epithelium. Species crossreactivity: human, rat, rabbit, hamster, canine, chicken, quail and porcine.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Human cytokeratins.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human cytokeratin 5. Stains basal cells in combined and stratified epithelial tissues. Recognizes the stem cell population, including the amplifying cell in the prostate epithelium. Species crossreactivity: human, rat, rabbit, hamster, canine, chicken, quail and porcine.
References
Smedts, F. et al. Changing patterns of keratin expression during progression of cervical intraepithelial neoplasia. Am. J. Path. (1990) 136:657–668.