Reactivos e instrumentos para inmunología, biología celular y biología molecular.
 
> DPTIP [351353-48-5]

DPTIP [351353-48-5]

Imprimir Imprimir
Por favor conéctese

Referencia HY-131002-10mg

embalaje : 10mg

Marca : MedChemExpress

Solicitar más información

Contact local distributor :


Teléfono : +1 850 650 7790

DPTIP is a potent brain penetrant neutral sphingomyelinase 2 (N-SMase 2) inhibitor (exosome inhibitor), with an IC50 of 30 nM.

Para uso exclusivo en investigación. No vendemos a pacientes.

DPTIP Estructura química

DPTIP Estructura química

No. CAS : 351353-48-5

This product is a controlled substance and not for sale in your territory.

Based on 1 publication(s) in Google Scholar

Ver todos los productos específicos de isoformas Phospholipase:

Ver todas las isoformas
Phospholipase A PLD Phospholipase C
Descripciòn

DPTIP is a potent brain penetrant neutral sphingomyelinase 2 (N-SMase 2) inhibitor (exosome inhibitor), with an IC50 of 30 nM[1][2].

In Vitro

DPTIP blocks EV secretion in a dose dependent manner (0.03-30 μM), and at 30 μM, this compound could decrease exosome release by 50% in astrocytes[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

DPTIP Related Antibodies
In Vivo

DPTIP potently (10 mg/kg IP) inhibits IL-1β-induced astrocyte-derived EV release[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Mice[1].
Dosage: 10 mg/kg.
Administration: IP 0.5 h prior to IL-1β striatal injection.
Result: Brain concentrations of DPTIP are above its IC50 for nSMase2 inhibition for at least 4 h after compound administration.
The number of astrocyte-derived EVs was reduced by 51 ± 13% 2 h post IL-1β administration.
Peso molecular

378.44

Fòrmula

C21H18N2O3S
No. CAS

351353-48-5
Appearance

Solid

Color

Off-white to gray

SMILES

COC1=CC(C2=NC(C3=CC=CC=C3)=C(N2)C4=CC=CS4)=CC(OC)=C1O
Envío

Room temperature in continental US; may vary elsewhere.
Almacenamiento
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvente y solubilidad
In Vitro: 

DMSO : 250 mg/mL (660.61 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.6424 mL 13.2121 mL 26.4243 mL
5 mM 0.5285 mL 2.6424 mL 5.2849 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Calculadora de molaridad

  • Calculadora de dilución

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: 2.08 mg/mL (5.50 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación

Purity: 99.68%

COA (255 KB) HNMR (277 KB) LCMS (96 KB)

Instrucciones de manejo (2659 KB)
Referencias

  • [1]. Camilo Rojas, et al. DPTIP, a newly identified potent brain penetrant neutral sphingomyelinase 2 inhibitor, regulates astrocyte-peripheral immune communication following brain inflammation. Sci Rep. 2018 Dec 7;8(1):17715.  [Content Brief]

    [2]. Huarui Zhang, et al. Advances in the discovery of exosome inhibitors in cancer. J Enzyme Inhib Med Chem. 2020 Dec;35(1):1322-1330.  [Content Brief]

  • [1]. Camilo Rojas, et al. DPTIP, a newly identified potent brain penetrant neutral sphingomyelinase 2 inhibitor, regulates astrocyte-peripheral immune communication following brain inflammation. Sci Rep. 2018 Dec 7;8(1):17715.

    [2]. Huarui Zhang, et al. Advances in the discovery of exosome inhibitors in cancer. J Enzyme Inhib Med Chem. 2020 Dec;35(1):1322-1330.

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.6424 mL 13.2121 mL 26.4243 mL 66.0607 mL
5 mM 0.5285 mL 2.6424 mL 5.2849 mL 13.2121 mL
10 mM 0.2642 mL 1.3212 mL 2.6424 mL 6.6061 mL
15 mM 0.1762 mL 0.8808 mL 1.7616 mL 4.4040 mL
20 mM 0.1321 mL 0.6606 mL 1.3212 mL 3.3030 mL
25 mM 0.1057 mL 0.5285 mL 1.0570 mL 2.6424 mL
30 mM 0.0881 mL 0.4404 mL 0.8808 mL 2.2020 mL
40 mM 0.0661 mL 0.3303 mL 0.6606 mL 1.6515 mL
50 mM 0.0528 mL 0.2642 mL 0.5285 mL 1.3212 mL
60 mM 0.0440 mL 0.2202 mL 0.4404 mL 1.1010 mL
80 mM 0.0330 mL 0.1652 mL 0.3303 mL 0.8258 mL
100 mM 0.0264 mL 0.1321 mL 0.2642 mL 0.6606 mL
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

DPTIP Related Classifications

  • Metabolic Enzyme/Protease
  • Phospholipase
Help & FAQs

Keywords:

DPTIP351353-48-5PhospholipaseexosomeN-SMaseextracellularvesiclesInhibitorinhibitorinhibit

Usted podría estar interesado también en los siguientes productos:



Referencia
Descripción
Cond.
Precio Sin IVA
HY-19363-10mg
GW4869 [6823-69-4]
 10mg 
HY-19363-25mg
GW4869 [6823-69-4]
 25mg