EnzyChrom™ Aldehyde Dehydrogenase Assay Kit
Application
- For quantitative determination of aldehyde dehydrogenase activity in biological samples (e.g serum, cell lysate, etc)
Key Features
- Sensitive and accurate. Linear detection range 0.2 – 25 U/L ALDH in a 96-well plate assay.
- Convenient. The assay involves the addition of a single working reagent and can be completed in under an hour.
- Robust and amenable to HTS. Homogeneous “mix-incubate-measure” type assay. No wash and reagent transfer steps are involved. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Method
- OD565nm
Samples
- biological samples (serum, cell lysate, etc)
Species
- All
Procedure
- 30min
Size
- 100 tests
Detection Limit
- 0.2 U/L
Shelf Life
- 6 months
More Details
ALDEHYDE DEHYDROGENASES (ALDHs) are a superfamily of oxidoreductases that catalyze the conversion of aldehydes to carboxylic acids. ALDH is crucial in the metabolism of alcohol as alcohol dehydrogenase breaks down ethanol to acetaldehyde. Acetaldehyde, which is toxic to the body, is in turn broken down by ALDH to acetic acid. Imbalances of ALDHs have been linked to both alcoholism and alcohol sensitivity in people.BioAssay Systems’ EADH-100 Kit is based on the enzymatic conversion of acetaldehyde to acetic acid and NADH by ALDH. The formed NADH is converted into a colored product, the absorbance of which, measured at 565 nm, is proportional to the enzyme activity in the reaction.Does the kit come with aldehyde dehydrogenase enzyme?
No, the user needs to provide their own purified enzyme.
I noticed that precipitate formed when I mixed the Working Reagent and cell lysate sample. Is this normal?
Yes, this should not be a problem. Try centrifuging the sample and using the clear supernatant to run the assay (e.g. 5 minutes at 14000 rpm).
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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.
– Fast turnaround
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