Western Blot (Transfected lysate)
Western Blot analysis of ERCC6L expression in transfected 293T cell line (H00054821-T02) by ERCC6L MaxPab polyclonal antibody.
Lane 1: ERCC6L transfected lysate(46.20 KDa).
Lane 2: Non-transfected lysate.
- Specifications
Product Description
Rabbit polyclonal antibody raised against a full-length human ERCC6L protein.
Immunogen
ERCC6L (AAH08808.1, 1 a.a. ~ 419 a.a) full-length human protein.
Sequence
MEKSFATKNEAVQKETLQEGPKQEALQEDPLESFNYVLSKSTKADIGPNLDQLKDDEILRHCNPWPIISITNESQNAESNVSIIEIADDLSASHSALQDAQASEAKLEEEPSASSPQYACDFNLFLEDSADNRQNFSSQSLEHVEKENSLCGSAPNSRAGFVHSKTCLSWEFSEKDDEPEEVVVKAKIRSKARRIVSDGEDEDDSFKDTSSINPFNTSLFQFSSVKQFDASTPKNDISPPGRFFSSQIPSSVNKSMNSRRSLASRRSLINMVLDHVEDMEERLDDSSEAKGPEDYPEEGVEESSGEASKYTEEDPSGETLSSENKSSWLMTSKPSALAQETSLGAPEPLSGEQLVGSPQDKAAEATNDYETLVKRGKELKECGKIQEALNCLVKALDIKSADPEVMLLTLSLYKQLNNN
Host
Rabbit
Reactivity
Human
Interspecies Antigen Sequence
Mouse (60); Rat (60)
Quality Control Testing
Antibody reactive against mammalian transfected lysate.
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
- Applications
Western Blot (Transfected lysate)
Western Blot analysis of ERCC6L expression in transfected 293T cell line (H00054821-T02) by ERCC6L MaxPab polyclonal antibody.
Lane 1: ERCC6L transfected lysate(46.20 KDa).
Lane 2: Non-transfected lysate.
- Gene Info — ERCC6L
Entrez GeneID
54821GeneBank Accession#
BC008808.2Protein Accession#
AAH08808.1Gene Name
ERCC6L
Gene Alias
FLJ20105, MGC131695, PICH
Gene Description
excision repair cross-complementing rodent repair deficiency, complementation group 6-like
Gene Ontology
HyperlinkGene Summary
O
Other Designations
Plk1-interacting checkpoint helicase|SNF2/RAD54 family protein|excision repair cross-complementing rodent repair deficiency complementation group 6 - like|excision repair protein ERCC6-like
- Interactomes
- Publication Reference
- Cytidine deaminase deficiency in tumor cells is associated with sensitivity to a naphthol derivative and a decrease in oncometabolite levels.
Hamza Mameri, Géraldine Buhagiar-Labarchède, Gaëlle Fontaine, Céline Corcelle, Caroline Barette, Rosine Onclercq-Delic, Claire Beauvineau, Florence Mahuteau-Betzer, Mounira Amor-Guéret.
Cellular and Molecular Life Sciences : CMLS 2022 Aug; 79(8):465.
Application：IF.
- Identification and Analysis of Different Types of UFBs.
Simon Gemble, Mounira Amor-Guéret.
Methods in Molecular Biology (Clifton, N.J.) 2020 Aug; 2153:187.
Application：IF, Human, Human adherent cell.
- A decrease in NAMPT activity impairs basal PARP-1 activity in cytidine deaminase deficient-cells, independently of NAD+.
Sandra Cunha Silveira, Géraldine Buhagiar-Labarchède, Rosine Onclercq-Delic, Simon Gemble, Elias Bou Samra, Hamza Mameri, Patricia Duchambon, Christelle Machon, Jérôme Guitton, Mounira Amor-Guéret.
Scientific Reports 2020 Aug; 10(1):13907.
Application：IF, Human, HeLa cells.
- Topoisomerase IIα Prevents Ultrafine Anaphase Bridges by Two Mechanisms.
Simon Gemble, Géraldine Buhagiar-Labarchède, Rosine Onclercq-Delic, Gaëlle Fontaine, Sarah Lambert, Mounira Amor-Guéret.
Open Biology 2020 May; 10(5):190259.
Application：IF, Human, HeLa cells.
- Chromosome alignment maintenance requires the MAP RECQL4, mutated in the Rothmund-Thomson syndrome.
Yokoyama H, Moreno-Andres D, Astrinidis SA, Hao Y, Weberruss M, Schellhaus AK, Lue H, Haramoto Y, Gruss OJ, Antonin W.
Life Science Alliance 2019 Feb; 2(1): e20180012.
Application：IF, Human, HeLa cells.
- 53BP1 can limit sister-chromatid rupture and rearrangements driven by a distinct ultrafine DNA bridging-breakage process.
Tiwari A, Addis Jones O, Chan KL.
Nature Communications 2018 Feb; 9:677.
Application：IF, Human, HeLa, U2OS, Saos-2 Cells.
- A role for Tau protein in maintaining ribosomal DNA stability and cytidine deaminase-deficient cell survival.
Bou Samra E, Buhagiar-Labarchède G, Machon C, Guitton J, Onclercq-Delic R, Green MR, Alibert O, Gazin C, Veaute X, Amor-Guéret M.
Nature Communications 2017 Sep; 8(1):693.
Application：IF, Human, HeLa cells.
- A balanced pyrimidine pool is required for optimal Chk1 activation to prevent ultrafine anaphase bridge formation.
Gemble S, Buhagiar-Labarchede G, Onclercq-Delic R, Biard D, Lambert S, Amor-Gueret M.
Journal of Cell Science 2016 Aug; 129(16):3167.
Application：IF, Human, HeLa cells.
- PTEN stabilizes TOP2A and regulates the DNA decatenation.
Kang X, Song C, Du X, Zhang C, Liu Y, Liang L, He J, Lamb K, Shen WH, Yin Y.
Scientific Reports 2015 Dec; 5:17873.
Application：IF, Human, Anaphase cells.
- A concomitant loss of dormant origins and FANCC exacerbates genome instability by impairing DNA replication fork progression.
Luebben SW, Kawabata T, Johnson CS, O'Sullivan MG, Shima N.
Nucleic Acids Research 2014 May; 42(9):5605.
Application：IS, Mouse, Embryonic fibroblasts.
- RING finger nuclear factor RNF168 is important for defects in homologous recombination caused by loss of the breast cancer susceptibility factor BRCA1.
Muñoz MC, Laulier C, Gunn A, Cheng A, Robbiani DF, Nussenzweig A, Stark JM.
The Journal of Biological Chemistry 2012 Nov; 287(48):40618.
Application：IF, Human, U2OS cells.
- Bloom's syndrome and PICH helicases cooperate with topoisomerase IIα in centromere disjunction before anaphase.
Rouzeau S, Cordelières FP, Buhagiar-Labarchède G, Hurbain I, Onclercq-Delic R, Gemble S, Magnaghi-Jaulin L, Jaulin C, Amor-Guéret M.
PLoS One 2012 Apr; 7(4):e33905.
Application：IF, WB, Human, BLM cells.
- The relative efficiency of homology-directed repair has distinct effects on proper anaphase chromosome separation.
Laulier C, Cheng A, Stark JM.
Nucleic Acids Res 2011 Mar; 39:5935.
Application：IF, Mouse, mES cells.
- Cytidine deaminase deficiency in tumor cells is associated with sensitivity to a naphthol derivative and a decrease in oncometabolite levels.