Fab Anti-MOUSE IgG (H&L) (RABBIT) Antibody
Referencia 810-4102
embalaje : 500ug
Marca : Rockland Immunochemicals
Specifications for Fab Mouse IgG (H&L) Antibody
Product Details
Rabbit Fab Anti-Mouse IgG (H&L) Antibody - 810-4102
Rabbit Fab Anti-Mouse IgG Antibody, Rabbit Fab Fragment Anti-Mouse IgG Antibody
Rabbit
IgG (H&L)
Polyclonal
IgG Fab
Target Details
Mouse
Mouse IgG whole molecule
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. No reaction was observed against anti-Papain or anti-Rabbit IgG F(c).
Application Details
IF - View References
Suitable for highly specific immunological methods requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Formulation
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Shipping & Handling
Wet Ice
Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Background
Fab Anti-Mouse IgG (H&L) Antibody generated in rabbit detects Mouse IgG. This product possesses the F(ab) region possessing the epitope-recognition site, both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
References (2)
Dembla et al. (2018). Early auto-immune targeting of photoreceptor ribbon synapses in mouse models of multiple sclerosis. EMBO Molecular Medicine
Applications
IF, Confocal Microscopy PubMed
Eich et al. (2017). The Calcineurin-Binding, Activity-Dependent Splice Variant Dynamin1xb Is Highly Enriched in Synapses in Various Regions of the Central Nervous System. Frontiers in Molecular Neuroscience
Applications
IF, Confocal Microscopy PubMed
Related Protocols
Immunocytochemistry (ICC) Protocol
Immunofluorescence (IF) Protocol
Certificate of Analysis Lookup
Disclaimer
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
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Technical Tips
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