GAPDH Monoclonal Antibody

Pedido mínimo 2

Referencia E-AB-48016-20

embalaje : 20uL

Marca : Elabscience

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Teléfono : +1 850 650 7790

Verified Samples Verified Samples in WB: DU145, OS-RC-2, T-47D, HEC-1B, HepG2, HEK-293, Rat heart, Porcine brain, Bovine kidney, Guinea pig liver, Rabbit pancreas, Caprine spleen
Verified Samples in IF: 293F
Dilution WB 1:5000-10000
Isotype IgG1
Host Mouse
Reactivity Human,  Mouse,  Rat ,  Bovine,  Caprine,  Guinea pig,  Rabbit,  Porcine,  
Applications WB,  IF
Clonality Monoclonal
Immunogen Recombinant human GAPDH protein expressed by E.coli
Abbre GAPDH
Synonyms BARS-38,   G3PDH,   GAPD,   KNC-NDS6,   OCAS,   cb609,   p38 component,  aging associated gene 9 protein
Swissprot
Calculated MW 36 kDa
Observed MW 36 kDa

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Cytoplasm, Cytoskeleton, Nucleus.
Concentration 1 mg/mL
Buffer PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4
Purification Method Protein A/G Purified
Research Areas Cancer,  Metabolism,  Signal Transduction
Clone No. 3B3
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended.
background GAPDH is an enzyme of 37kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. Because the GAPDH gene is often stably and constitutively expressed at high levels in most tissues and cells, it is commonly used as a loading control for western blot and as a control for qPCR.
Cat.No. Product Name Sizes
E-AB-1001 Goat Anti-Mouse IgG(H+L)(peroxidase/HRP conjugated) 500μL , 120μL , 60μL
E-AB-1011 Goat Anti-Mouse IgG(H+L)(Cyanine3 conjugated) 500μL , 120μL , 60μL
E-AB-1015 Goat Anti-Mouse IgG(H+L)(FITC conjugated) 500μL , 120μL , 60μL
E-AB-1024 Goat Anti-Mouse IgG(H+L)(Biotin conjugated) 500μL , 120μL , 60μL
E-AB-1043 Streptavidin(peroxidase/HRP conjugated) 500μL , 120μL , 60μL
E-AB-1059 Goat Anti-Mouse IgG(H+L)(Elab Fluor® 594 conjugated) 500μL , 120μL , 60μL
E-AB-1076 Goat Anti-Mouse IgG(H+L)(Elab Fluor® 647 conjugated) 500μL , 120μL , 60μL
E-AB-1196 HRP-Goat Anti-Mouse IgG(H+L) preadsorbed 500μL , 120μL , 1mL
E-IR-R217 2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution) 50mL , 18mL , 6mL , 3mL
E-IR-R220 Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH9.0) 10mL , 3mL
E-IR-R221 Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH6.0) 10mL , 3mL
E-IR-R304A Western Blot Detection Kit 50Assays
E-IR-R304B High Accuracy and Absorbability Western Blot Detection Kit 50Assays
Other Clones

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Elab Fluor®488

Elab Fluor®594

HRP

Unconjugated

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