K2229-100 assays | Glutamate Dehydrogenase Activity Colorimetric Assay

Background

Glutamate dehydrogenase (GDH) is an enzyme that converts glutamate to α-ketoglutarate and is present in the mitochondria of eukaryotes and most microbes. Since increased serum GDH levels indicate liver damage, GDH often used as an indicator of liver disease, often in combination with aminotransferases.

The Glutamate Dehydrogenase Activity Colorimetric Assay Kit provides a sensitive, simple, fast and convenient way for detection of GDH activity in various samples based on colorimetric method. In the assay, GDH in sample consumes glutamate as a specific substrate and produces NADH stoichiometrically, resulting in a proportional color development that can be easily quantified colorimetrically (λmax = 450 nm). The kit can detect GDH activity as low as 0.01 mU in serum or tissue and cell extracts.

Features & Properties

Features	Fast and convenient; Simple procedure; Sensitive assays for measuring GDH activity in various biological samples and the kit is also suitable for high throughput analyses.
Shipping	Blue Ice
Storage Conditions	Store at -20°C.

Quality Control

DataSheet

Storage

Store at -20°C.

Overview

Kit components

GDH Assay Buffer, Glutamate (2 M), GDH Developer, GDH Positive Control, NADH (0.5 μmol)

Glutamate Dehydrogenase Activity Colorimetric Assay Kit

Referencia K2229-100 assays

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