Grb2 (Growth Factor Receptor-Bound Protein 2, ASH, Abundant Src Homology) (Biotin)

Gab2 (Grb2-associated binder) is the latest member of a new family of multi-functional adapter molecules containing a Pleckstrin Homology domain, defined initially by the cloning of Gab1. Gab2 is broadly expressed as a 90-100kD protein. It contains two putative SH3 binding motifs and is tyrosine phosphorylated in response to a range of cytokine, growth factor, and antigen stimulation. Gab2 associates with SHP2, the p85 subunit of PI3K, Grb2, and SHC demonstrating its involvement in multiple signaling pathways. Gab 2 transfection experiments show that Gab2, like Gab1, stimulates Erk kinase activity following EGF-stimulation or co-transfection with activated Ras. By contrast, while Gab1 increases activated Ras or EGF-mediated transactivation of Ekl-1 responsive genes, Gab2 has an inhibitory effect. Thus, Gab2 may function as a negative regulator of cytoplasmic signaling to the nucleus. ||Applications:|Suitable for use in Western Blot. Not recommended for Immunoprecipitation. Other applications not tested. ||Recommended Dilution:|Western Blot: 0.5-2mg/ml. Detects GRB2 (23–25kD) in RIPA lysates of non-stimulated human A431 carcinoma cells. Previous lots detected GRB2 in RIPA lysates of mouse 3T3 fibroblasts, rat L6 myoblasts, chinese hamster ovary cells and bovine brain extract. |Optimal dilutions to be determined by the researcher. ||Recommended Secondary Antibodies:|I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo|I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo||Positive Antigen Control (Included): |A0001-50: A431 Cells, Non-Stimulated in RIPA Buffer, Sample Buffer.||Storage and Stability:|Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. ||Note: Applications are based on unconjugated antibody.