This kit is based on indirect ELISA detection method and takes 3h assay time. The microplate provided in this kit has been precoated with antigen. HRP-antibody is used as detection antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add HRP-detection antibody, then it binds with anti-SARS-CoV2 (N) IgG bound to precoated antibody. Wash unbound components and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of anti-SARS-CoV2 (N) IgG in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.
Manual- Product Name
- Human anti-SARS-CoV2(N) IgG ELISA Kit
- Alias
- SARS-CoV-2(N) IgG ELISA Kit
- Catalogue No.
- EH4397
- Size
- 48T/96T
- Species
- Human
- Sample Type
- Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
- Detection Method
- Indirect ELISA
- Detection Wavelength
- OD450
- Reaction Duration
- 1.5 hours
- Range
- 3.906-250ng/ml
- Sensitivity
- 2.344ng/ml
- Storage
- 2-8°C(Sealed), Don't cryopreserve.
- Specificity
- Specifically binds with anti-SARS-CoV2 (N) IgG , no obvious cross reaction with other analogues.
- Required Instruments and Reagents
-
- Microplate reader (wavelength: 450nm)
- 37°C incubator (CO2 incubator for cell culture is not recommenced.)
- Automated plate washer or multi-channel pipette/5ml pipettor (for manual washing purpose)
- Precision single (0.5-10μL, 5-50μL, 20-200μL, 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
- Sterile tubes and Eppendorf tubes with disposable tips
- Absorbent paper and loading slot
- Deionized or distilled water
- Assay Procedure Summary
-
- Standard Curve
-
This product is detected by QC department and meets performance required in the manual. (Laboratory Humidity: 20%-60%; Temperature: 18°C -25°C; Equilibrate TMB substrate to 37°C before staining. After adding into the ELISA wells, incubate for 15min at 37°C in dark.)
Due to different assay environments and operations, assay data below and standard curve are provided for reference. Experimenters should establish standard curve according to their own assay.
STD.(ng/ml) OD-1 OD-2 Average 0 0.05 0.053 0.051 3.906 0.203 0.213 0.207 7.813 0.336 0.353 0.343 15.625 0.554 0.583 0.566 31.25 0.9 0.945 0.918 62.5 1.246 1.309 1.271 125 1.719 1.807 1.754 250 2.05 2.154 2.092 - Recovery
-
Add a certain amount of anti-SARS-CoV2 (N) IgG into the sample. Calculate the recovery by comparing the measured value with the expected amount of anti-SARS-CoV2 (N) IgG in the sample.
Sample Type Recovery Range(%) Average(%) serum(n=10) 88-104 96 EDTA plasma(n=10) 87-103 93 Heparin plasma(n=10) 87-105 95 - Linearity
-
Dilute the sample with a certain amount of anti-SARS-CoV2 (N) IgG at 1:2, 1:4 and 1:8 to get the recovery range.
Sample Type 1:2 1:4 1:8 serum(n=10) 85-97% 86-98% 86-104% EDTA plasma(n=10) 84-101% 85-98% 91-98% Heparin plasma(n=10) 88-100% 83-97% 80-97% - Precision(%)
-
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on the same plate.
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
Item Intra-assay Precision Inter-assay Precision Sample 1 2 3 1 2 3 n 20 20 20 20 20 20 Mean (ng/ml) 7.3 29.22 123.12 7.67 29.93 124.1 Standard deviation 0.32 1.3 5.76 0.34 1.37 5.78 CV(%) 4.43 4.46 4.68 4.37 4.59 4.66 - Stability
-
Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.
ELISA kit(n=5) 37°C for 1 month 2-8°C for 6 months Average(%) 80 95-100
- Journal:
- Nature Communications
- Cited Date:
- 2022-02-18
- Product:
-
- Human anti-SARS-CoV2(N) IgG ELISA Kit
- Journal:
- The Journals of Gerontology: Series A
- Cited Date:
- 2021-07-23
- Product:
-
- Human anti-SARS-CoV2(S) IgG ELISA Kit
- Human anti-SARS-CoV2(N) IgG ELISA Kit
- Journal:
- Healthcare
- Author:
- Department of Human Motor Skills, Faculty of Physical Education and Sport, Wroclaw University of Health and Sport Sciences, 51-612 Wroc?aw, Poland
- Cited Date:
- 2023-10-27
- Product:
-
- Human anti-SARS-CoV2(N) IgG ELISA Kit
- Journal:
- Journal of Integrative and Complementary Medicine
- Cited Date:
- 2022-09-22
- Product:
-
- Human anti-SARS-CoV2(N) IgG ELISA Kit
- Journal:
- Research Square
- Author:
- Central University Hospital of Asturias
- Sample:
- serum
- Cited Date:
- 2024-08-30
- Product:
-
- Human anti-SARS-CoV2(S) IgG ELISA Kit
- Human anti-SARS-CoV2(N) IgG ELISA Kit
- Journal:
- Polish Annals of Medicine
- Author:
- Department of Biochemistry, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Poland
- Cited Date:
- 2023-10-20
- Product:
-
- Human anti-SARS-CoV2(N) IgG ELISA Kit