96 Tests PN: B113336

Components:

45x Ab-conjugated beads (S5P5-human sEPOR Ab-bead). PN: B113336A. One vial containing 100 P5.

25x Biotin-detection (human sEPOR Biotin-dAb). PN: B113336B. One vial containing 100 Panel B, 9-Plex. PN: HG9009B. One vial containing lyophilized recombinant human sCEACAM-1, sALCAM, sSLAMF4, sHVEM, sB7-DC, sB7-H1, sEpCAM, sBLAME and sEPOR.  Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

Application: Optimal antibody pair and antigen standard for assaying human Human EpoR/Erythropoietin Receptor. Can be multiplexed with other analytes in Human Group 9.  To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 20 pg/mL

  • Quantitation range:

  • LLOQ: < 450 pg/mL

  • ULOQ: > 10,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Cross-reactivity of analytes in Human Group 9: Negligible

  • Sample volume: 15 ). "Crystallographic evidence for preformed dimers of erythropoietin receptor before ligand activation.". Science 283 (5404): 987. doi:10.1126/science.283.5404.987. PMID 9974392.

  • Youssoufian H, Longmore G, Neumann D, Yoshimura A, Lodish HF (May 1993). "Structure, function, and activation of the erythropoietin receptor". Blood 81 (9): 2223. PMID 8481505.

  • Wilson IA, Jolliffe LK (December 1999). "The structure, organization, activation and plasticity of the erythropoietin receptor". Curr. Opin. Struct. Biol. 9 (6): 696. doi:10.1016/S0959-440X(99)00032-9. PMID 10607675.

  • Farrell F, Lee A (2004). "The erythropoietin receptor and its expression in tumor cells and other tissues". Oncologist. 9 Suppl 5: 18. doi:10.1634/theoncologist.9-90005-18. PMID 15591419.