Human IL-8 ELISA Kit (48-well)
Referencia EA100031
embalaje : 1x48wells
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| Product Data | |
| Species | Human |
|---|---|
| Format | 48-well strip plate |
| Assay Type | Solid Phase Sandwich ELISA |
| Assay Length | 3 hours |
| Signal | Colorimetric |
| Curve Range | 15.625-1000pg/ml |
| Sample Type | Cell culture supernatant, serum, plasma (EDTA, citrate, heparin) |
| Sample Volume | 20 uL |
| Specificity | Natural and recombinant Human IL-8 Ligand |
| Sensitivity | 7pg/mL |
| Reactivity | Human |
| Interference | No significant interference observed with available related molecules. |
| Components |
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| Background | Interleukin 8 (IL-8), a member of the neutrophil-specific CXC subfamily of chemokines, is a potent neutrophil chemotactic and activating factor. It is a primary inflammatory cytokine produced by many cells including monocytes/ macrophages, T cells, neutrophils, fibroblasts, endothelial cells, keratinocytes, hepatocytes, astrocytes and chondrocytes. It is in response to proinflammatory stimuli such as IL-1, TNF, LPS and viruses. Its function is, in part, to attract neutrophils to the site of inflammation and to activate them. The human IL-8 cDNA sequence predicts a protein of 99 amino acids. Removal of a 22-residue signal peptide generates a mature protein of 77 amino acids (~ 8 kDa). Further proteolysis of the N-terminal end leads to a variant form with 72 amino acids; full activation of IL-8 may require cleavage to the 72 amino acid form. IL-8 can form non-covalent dimers in solution, especially at high concentrations, but dimerization is not necessary for biological activity. IL-8 binds to two seven-transmembrane, G protein-coupled receptors, CXCR1 and CXCR2, as well as to the non-signalling Duffy antigen on red-blood cells. The Duffy antigen may play a role in regulating IL-8 activity on functional receptors. |
| Gene Symbol | CXCL8 |
| Locus ID | 3576 |
| Standard Curve |  |
| Shipping | Blue Ice |
| Datasheet | |
| SDS | |