Human Insulin ELISA kit

Referencia EA100870

embalaje : 1x96wells

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Human Insulin ELISA kit

Specifications
Product Data
Assay Length 3 hours
Assay Platform 96-well plate
Assay Type Solid Phase Sandwich ELISA
Assay Type Sandwich
Background Insulin is the principal hormone responsible for the control of glucose metabolism. It is synthesized in the β- cells of the islets of Langerhans as the precursor, proinsulin, which is processed to form C-peptide and insulin. Both are secreted in equimolar amounts into the portal circulation. The mature insulin molecule comprises two polypeptide chains, the A chain and B chain (21 and 30 amino acids respectively). The two chains are linked together by two inter-chain disulphide bridges. There is also an intra-chain disulphide bridge in the A chain. Insulin concentrations are severely reduced in insulin-dependent diabetes mellitus (IDDM) and some other conditions such as hypopituitarism. Insulin levels are raised in non-insulin- dependent diabetes mellitus (NIDDM), obesity, insulinoma and some endocrine dysfunctions such as Cushing’s syndrome and acromegaly.
Components
  • 1. Microwell coated with Streptavidin: 12x8x1
  • 2. Insulin Standards: 6 vials ( ready to use): 0.5ml
  • 3. Insulin Conjugate reagent: 1 vial: 12 ml
  • 4. TMB Substrate: 1 bottle (ready to use): 12ml
  • 5. Stop Solution: 1 bottle (ready to use): 12ml
  • 6. 20X Wash concentrate: 1 bottle: 25ml
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Curve Range 2.6 -66 pmol/l
Format 8x12 divisible strips
Interference No significant interference observed with available related molecules.
Reactivity Human
Reactivity Human
Reactivity Human
Sample Type Serum
Sample Volume 25µl/well
Sensitivity 2.6 pmol/l
Signal Colorimetric
Species Human
Specificity This kit is used for quantitative detection human Insulin
New No
Description Human Insulin ELISA kit

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