Human Isocitrate dehydrogenase (IDH1) activation kit by CRISPRa
Referencia GA102310
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Human Isocitrate dehydrogenase (IDH1) activation kit by CRISPRa
SKU
GA102310
IDH1 CRISPRa kit - CRISPR gene activation of human isocitrate dehydrogenase (NADP(+)) 1, cytosolic
2 Weeks*
| Product Data | |
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
|---|---|
| Target Symbol | IDH1 |
| Locus ID | 3417 |
| Components | GA102310G1, Isocitrate dehydrogenase gRNA vector 1 in pCas-Guide-GFP-CRISPRa GA102310G2, Isocitrate dehydrogenase gRNA vector 2 in pCas-Guide-GFP-CRISPRa GA102310G3, Isocitrate dehydrogenase gRNA vector 3 in pCas-Guide-GFP-CRISPRa 1 CRISPRa-Enhancer vector, SKU GE100056 1 CRISPRa scramble vector, SKU GE100077 |
| Disclaimer | These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc. |
| Reference Data | |
| RefSeq | NM_001282386, NM_001282387, NM_005896, N21575 |
| UniProt ID | O75874 |
| Synonyms | HEL-216; HEL-S-26; IDCD; IDH; IDP; IDPC; PICD |
| Summary | Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Sep 2013] |
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| SDS |
| SKU | Description | Size | ||
|---|---|---|---|---|
| KN210582 | IDH1 - human gene knockout kit via CRISPR, HDR mediated | 1 kit | | |
| KN210582BN | IDH1 - human gene knockout kit via CRISPR, HDR mediated | 1 kit | | |
| KN210582LP | IDH1 - human gene knockout kit via CRISPR, HDR mediated | 1 kit | | |
| KN210582RB | IDH1 - human gene knockout kit via CRISPR, HDR mediated | 1 kit | | |
| KN410582 | IDH1 - KN2.0, Human gene knockout kit via CRISPR, non-homology mediated. | 1 kit | | |
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.