600173 | Human TNF-alpha Quimigen Portugal

Recombinant Active Protein

Cat. No.

600173

Unit

0.05 mg

Description

TNF (Tumor necrosis factor) is secreted by macrophages, monocytes, neutrophils, T-cells, and NK-cells following their stimulation by bacterial LPS. Cells expressing CD4 secrete TNF-alpha while CD8 cells secrete little or no TNF-alpha. The synthesis of TNF-alpha is induced by many different stimuli including interferons, IL2, and GM-CSF. Genetic variations in TNF are associated with susceptibility to psoriasis and hepatitis B virus infection. Recombinant Human Tumor Necrosis Factor-alpha produced in E. coli is a single non-glycosylated polypeptide chain containing 158 amino acids with a MW of 17,483.77 Da.

Spec Container

Datasheet

Format

Each vial contains 50 ug protein lyophilized in 10 mM sodium phosphate pH8.0, 20 mM NaCl. Reconstitute with 0.5 ml sterile deionized water for a final concentration of 0.1 mg/ml.

Alternate Names

tumor necrosis; TNF- alpha; tumor necrosis factor ligand superfamily member 2; TNF-a; cachectin; tumor necrosis factor, membrane form; tumor necrosis factor, soluble form

Accession No.

P01375

17484 Da

Sequence

VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELRDNQLVV PSEGLYLIYS QVLFKGQGCP STHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINRPD YLDFAESGQV YFGIIAL

Source

E. coli. Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.

Purity

Purity > 98% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.

Application Notes

Recombinant Tumor Necrosis Factor-alpha is fully biologically active when compared to standard. The ED50 as determined by the cytolysis of mouse L929 cells in the presence of Actinomycin D is less than 0.05 ng/ml.

Storage

Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.

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Citations

Product Citations

Hsu DSS et al. 2014. Cancer Cell. 26(4): 534-48. PMID 25314079

For research use only, not for diagnostic or therapeutic procedures.

Human TNF-alpha

Referencia 600173

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Marca : Abbiotec

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Spec Container