Purified Human Type V Collagen (lyophilized) for use as a coating material and standard.
ELISA plate was coated with serially diluted Human Type V Collagen (SB Cat. No. 1270-01). Purified collagen was detected with Goat Anti-Type I Collagen-BIOT (SB Cat. No. 1310-08), Goat Anti-Type II Collagen-BIOT (SB Cat. No. 1320-08), Goat Anti-Type III
Collagen is the main structural protein in the extracellular space and is the most abundant protein in the ECM. Collagens are divided into two classes - fibril (types I, II, III, V) and non-fibril (types IV, VI). Type V collagen is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen mutations are associated with Ehlers-Danlos syndrome. Type V collagen is broadly expressed as a two α1(V) chains and one α2(V) chain heterotrimer but also as a α1(V), α2(V), and α3(V) heterotrimer in pancreatic islets, adipose tissue, and skeletal muscle.
Source
Placental villi
Purity
> 90% by SDS-PAGE
Purification Method
Controlled and limited pepsin digestion followed by selective salt precipitation
Buffer Formulation
Lyophilized from 500 mM acetic acid
Recommended Storage
2-8-3 SDS-PAGE Coating Material for -3 ECM Interaction Studies ,6
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