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> IgA, alpha (HRP)

IgA, alpha (HRP)

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Referencia I1890-05M-1mg

embalaje : 1mg

Marca : US Biological

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I1890-05M IgA, alpha (HRP)

Clone Type
Polyclonal
Host
goat
Source
rat
Isotype
IgG
Grade
Affinity Purified
Applications
E IHC WB
Crossreactivity
Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C

Applications: |Suitable for use in Western Blot, ELISA and Immunohistochemistry. Other applications have not been tested.||Recommended Dilutions: |ELISA: 1:150,000|Western Blot: 1:2000-1:10,000|Immunohistochemistry: 1:1000-1:5000|Optimal dilutions to be determined by the researcher||Storage and Stability:|Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Conjugated to horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.

Applications
Product Type: Pab|Isotype: IgG|Host: goat|Source: rat|Concentration: ~1mg/ml (after reconstitution)|Form: Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% thimerasol. Labeled with horseradish peroxidase (HRP) Reconstitute with 1ml sterile ddH2O.|Purity: Purified by immunoaffinity chromatography.|Immunogen: Rat IgA, alpha chain|Specificity: Recognizes rat IgA, alpha chain. Specificity was confirmed by ELISA at <1% crossreactivity against other rat heavy or light chain isotypes. Assay by IEP resulted in a single precipitin arc against anti-peroxidase, anti-goat serum, rat IgA and rat serum.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Rat IgA, alpha chain
Form
Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% thimerasol. Labeled with horseradish peroxidase (HRP) Reconstitute with 1ml sterile ddH2O.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes rat IgA, alpha chain. Specificity was confirmed by ELISA at <1% crossreactivity against other rat heavy or light chain isotypes. Assay by IEP resulted in a single precipitin arc against anti-peroxidase, anti-goat serum, rat IgA and rat serum.
References
1. Farr & Nakane, J. Immunol. Methods 47: 129-144 (1981).