IgG, Kappa (Biotin)

Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)||Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule||Application(s): immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assay using streptavidin or avidin conjugates requiring lot-to-lot consistency.||Recommended Dilution(s): This product has been assayed against 1ug of Mouse Kappa in a standard capture ELISA using Peroxidase Conjugated Streptavidin. A working dilution of 1:10,000 to 1:20,000 of the reconstitution concentration is suggested for this product. Optimal titers for other applications should be determined by the researcher.||Storage Conditions: |Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.