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> Indole-3-butyric acid [133-32-4]

Indole-3-butyric acid [133-32-4]

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Referencia HY-N0186-5g

embalaje : 5g

Marca : MedChemExpress

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Descripciòn

Indole-3-butyric acid (3-indolebutyric acid) is a plant growth auxin and a good rooting agent. It can promote herbs and woody ornamental plant rooting and used for improving fruit rate. Indole 3-butyric acid is an auxin precursor, and is converted to indole 3-acetic acid (IAA) in a peroxisomal β-oxidation process[1].

IC50 & Target

Microbial Metabolite

 

Human Endogenous Metabolite

 

In Vitro

Indole-3-butyric acid (10 μM) induces adventitious root (AR) formation in the thin cell layers (TCLs)[2].
Indole-3-butyric acid (1 μM) induces lateral root formation by the promotion of NO production in Arabidopsis[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Indole-3-butyric acid Related Antibodies

Solvente y solubilidad
In Vitro: 

DMSO : ≥ 35 mg/mL (172.21 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.9203 mL 24.6015 mL 49.2029 mL
5 mM 0.9841 mL 4.9203 mL 9.8406 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (12.30 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.
Pureza y Documentación
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