Magnesium (Mg) Colorimetric Assay Kit

Referencia E-BC-K162-S-50

embalaje : 50Assays

Marca : Elabscience

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Teléfono : +1 850 650 7790

Detection Principle The magnesium in the serum reacts with the complexometric indicator (Calmagite) to form the Calmagite-Mg compound. The absorbance of this compound at 540 nm is proportional to the concentration of magnesium in the sample. The concentration of magnesium can be calculated by measuring the OD value at 540 nm.
Synonyms Mg
Sample Type Serum,Plasma
Detection Method Colorimetric method
Detection Instrument Spectrophotometer (540 nm)
Research Area Inorganic Ions ,  Plant Physiology Series
Other Reagents Required Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4)
Storage This product can be stored at 2-8°C for 12 months with shading light.
Valid Period 12 months
Sensitivity 0.12 mmol/L
Detection Range 0.12-2.50 mmol/L
Precision inter-assay CV: 9.9% | intra-assay CV: 4.8%
Assay Time 50 min

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type Dilution Factor
Human serum 1
Rat serum 1
Mouse serum 1
Porcine serum 1
Chicken serum 1
Dog serum 1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.

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