CD16 Unconjugated
Catalog #
Size
Lymphocyte-gated PBMCs stained with unconjugated CD16 followed by goat anti-mouse IgG Alexa Fluor 488 (RED); Lymphocyte-gated PBMCs stained with unconjugated mouse IgG1 followed by goat anti-mouse IgG Alexa Fluor 488 (BLUE).
| Conjugation | Unconjugated |
|---|---|
| Antigen | CD16 |
| Clone | 3G8 |
| Isotype | IgG1, k |
| Applications | FC |
| Reactivity | Baboon, Cynomolgus monkey, Human, Rhesus |
| Host | Mouse |
| Clonality | Monoclonal |
| Primary/Secondary | Primary |
| Immunogen | Human peripheral blood T lymphocytes |
| Storage | 2-8°C |
| Status | RUO |
Description:
CD16 (3G8) binds to the 50-65 kDa transmembrane protein of IgG FcγRIII, an surface antigen commonly associated with human NK cells. Besides surface expression on NK cells, it is also expressed on activated monocytes, macrophages, on neutrophils and placental trophoblasts in humans. CD16 binds aggregated IgG or IgG-antigen complex which functions in NK cell activation, phagocytosis, and antibody-dependent cell-mediated cytotoxicity (ADCC). 3G8 is also useful in experiments related to immunohistochemical staining, immunoprecipitation, NK cell proliferation, blocking of phagocytosis and blocking of immunoglobulin binding tthrough FcγRIII.
CD16 (3G8) binds to the 50-65 kDa transmembrane protein of IgG FcγRIII, an surface antigen commonly associated with human NK cells. Besides surface expression on NK cells, it is also expressed on activated monocytes, macrophages, on neutrophils and placental trophoblasts in humans. CD16 binds aggregated IgG or IgG-antigen complex which functions in NK cell activation, phagocytosis, and antibody-dependent cell-mediated cytotoxicity (ADCC). 3G8 is also useful in experiments related to immunohistochemical staining, immunoprecipitation, NK cell proliferation, blocking of phagocytosis and blocking of immunoglobulin binding tthrough FcγRIII.