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> Mouse CTSD (Cathepsin D) CLIA Kit

Mouse CTSD (Cathepsin D) CLIA Kit

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Referencia E-CL-M0180-96T

embalaje : 96T

Marca : Elabscience

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Product Summary
Sensitivity 7.5 pg/mL
Detection Range 12.5-800 pg/mL
Sample Volume 100 μL
Total Assay Time 3 h 30 min
Reacitivity Mouse
Specificity This kit recognizes Mouse CTSD in samples.No significant cross-reactivity or interference between Mouse CTSD and analogues was observed
Recovery 80%-120%
Sample Type Serum, plasma and other biological fluids
Detection Method Chemiluminescence
Assay Type Sandwich-ELISA
Size 96T / 48T / 24T / 96T*5 / 96T*10
Storage 2-8℃/-20℃
Expiration Date 6 months
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse CTSD. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CTSD and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CTSD, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse CTSD. You can calculate the concentration of Mouse CTSD in the samples by comparing the RLU value of the samples to the standard curve.
Uniport ID P18242
Research Area Cancer , Cell Biology , Neuroscience , Signal Transduction
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