Mouse IgA Antibody : PE

Referencia OASB00193

embalaje : 0.2mg

Marca : Aviva Systems Biology

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Mouse IgA Antibody : PE (OASB00193)

Datasheets/ManualsPrintable datasheet for Mouse IgA Antibody : PE (OASB00193)
Product Info
Tested Species ReactivityMouse
Predicted Species ReactivityMouse
ClonalityMonoclonal
Clone11-44-2
IsotypeIgG1
HostRat
ConjugationR-PE
ApplicationFC, FLISA
Reconstitution and StorageStore at 2-8C
Concentration0.1 mg/mL
SpecificityIgA
CharacterizationTo ensure lot- to- lot consistency, each batch of monoclonal antibody is tested by ELISA and/or flow cytometryto conform to characteristics of a standard reference reagent.
WarningReagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash withcopious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water before discardingto avoid accumulation of potentially explosive deposits in lead or copper plumbing.
DilutionFlow Cytometry:
ELISA:
Fluorescein conjugate
Biotin conjugate
R-phycoerythrin conjugate
Alkaline phosphatase conjugate
Horseradish peroxidase conjugate
<=1 ug/106 cells
<=1 ug/106 cells
<=0.2 ug/106 cells
1:500-1:1,000
1:1,000-1:2,000
Application InfoIdentification and enumeration of IgM+ cells by flow cytometry, Identification and enumeration of IgM+ cells by immunofluorescence microscopy, Second step reagent for mouse IgM monoclonal antibodies, Enzyme-Linked-Immunosorbent-Assay (ELISA)
Other Applications DataSince applications vary, each investigator should determine the optimum working dilutions of the product that is appropriate for their specificneeds.
Storage- The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C
- The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C
- The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mMMgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2- 8 C or long- term at - 20 C
- The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50%PBS, pH 7.4. No preservative added. Store at 2- 8 C or long- term at - 20 C
- The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C
- The R- phycoerythrin (R- PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3and a stabilizing agent. Store at 2- 8 C. Do not freeze!
- Protect conjugated forms from light. Reagents are stable for the period shown on the label if stored asdirected.
Gene SymbolIGHA
Gene Full NameB-cell antigen receptor complex-associated protein alpha chain
Alias SymbolsI, IgA, Igh-, Igh-2
NCBI Gene Id238447
Uniprot IDA0A0A6YXW6