Mouse IL-2 ELISA Kit (96-well)
Referencia EA100120
embalaje : 1x96wells
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| Product Data | |
| ACCN | NM_008366 |
|---|---|
| Assay Length | 3 hour |
| Assay Platform | 96-well plate |
| Assay Type | Solid Phase Sandwich ELISA |
| Assay Type | Sandwich |
| Background | Interleukin 2 (IL-2) is a pleiotropic cytokine produced primarily by mitogen- or antigen-activated T lymphocytes . Human IL-2 (also known as T-cell growth factor) is produced by T-cells in response to antigenic or mitogenic stimulation. IL-2 is a potent lymphoid cell growth factor which exerts its biological activity primarily on T cells promoting proliferation and maturation. Additionally, IL-2 has been found to stimulate growth and differentiation of B cells, NK cells, LAK cells, monocytes, and oligodendocytes. IL-2 is involved in treatment of cancers such as melanoma and renal cell cancer. It plays a key role in promoting the clonal expansion of antigen-specific T cells. In addition, IL-2 has also been shown to mediate multiple immune responses on a variety of cell types. The sequence of mouse IL-2 cDNA predicts a 169 amino acid (aa) residue precursor glycoprotein containing a 20 aa residue signal peptide that is cleaved to form the mature protein . At the amino acid sequence level, mature mouse IL-2 is approximately 60% identical to human IL-2 . Whereas human IL-2 is active on mouse cells, mouse IL-2 is species-specific and is inactive on human cells . The gene for IL-2 has been mapped to mouse chromosome 3 . The biological effects of IL-2 are mediated by specific cell surface receptor complexes. The functional high-affinity receptor for IL-2 is composed of three distinct polypeptide chains . IL-2 stimulates the proliferation of thymocytes; stimulates the proliferation and differentiation of activated B cells; promotes the growth, differentiation and cytocidal activity of monocytes; induces the growth of natural killer cells and stimulates cytokine production by these cells as well as the cytolytic activity of these cells; enhances the production of lymphocyte-activated killer (LAK) cells; and induces the proliferation and differentiation of oligodendrocytes . |
| Components |
|
| Curve Range | 0.02 - 1.0 ng/mL |
| figure_legend | Representative standard curve for IL-2 ELISA. IL-2 was diluted in serial two-fold steps in Sample Diluent. |
| Format | 96-well strip plate |
| Gene Symbol | Il2 |
| Interference | No significant interference observed with available related molecules. |
| Locus ID | 16183 |
| Reactivity | Mouse |
| Reactivity | Mouse |
| Reactivity | Mouse |
| Sample Type | Cell culture supernatant, serum, plasma (EDTA, citrate, heparin) |
| Sample Volume | 20 uL |
| Sensitivity | 7pg/mL |
| Signal | Colorimetric |
| Species | Mouse |
| Specificity | Natural and recombinant Mouse IL-2 Ligand |
| Standard Curve | /assets/images/elisa/kits/128/cme0001-096-1-e.jpg |
| Target Symbol | Il2 |
| Description | Mouse IL-2 ELISA Kit (96-well) |