Mouse Monoclonal Anti-AAV2 (intact particle), lyophilized, purified [Clone A20]
Referencia 61055
embalaje : 50µg
Marca : Progen
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Teléfono : +1 850 650 7790
| Quantity | 50 µg |
|---|---|
| Antibody Type | Monoclonal |
| Host | Mouse |
| Isotype | IgG3 |
| Conjugate | Unconjugated |
| Application | Affinity chromatography, Dot blot, ELISA, ICC/IF, IP, Neutralization assay |
| Purification | Affinity chromatography |
| Reactivity | AAV2, AAV2 7m8, AAV3 |
| No reactivity | AAV1, AAV4, AAV5, AAV6, AAV7, AAV8, AAV9, AAV11, AAV12, AAVDJ, AAVrh10, AAVrh74 |
| Storage before reconstitution | 2-8°C until indicated expiry date |
| Storage after reconstitution | 2-8°C |
| Intended use | Research use only |
| Clone | A20 |
| Immunogen | AAV2 capsids |
| Concentration | 50 µg/ml after reconstitution with 1 ml dist. water |
| Formulation | Lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4) |
| Binding affinity | KD value (AAV2) = 2.6E-11 M KD value (AAV3) = <1.0E-12 M |
| Synonym | Adeno-associated virus 2; AAV-2 |
| Tested applications | Tested dilutions |
|---|---|
| Immunocytochemistry (ICC)/ Immunofluorescence (IF) | 1:20 |
| Affinity Chromatography | Assay dependent |
| Dot Blot | 1:500 (0.1 µg/ml; non-denaturing conditions) |
| ELISA | Assay dependent |
| Immunoprecipitation (IP) | 1:5 |
| Neutralization Assay | EC50 ~5 ng/ml (AAV2) and ~3 ng/ml (AAV3) - assay dependent |
For characterization of different stages of infection and very useful for the analysis of the AAV2 assembly process. A20 specifically reacts with AAV2 and AAV3, empty and full capsids. Recognizes a conformational epitope of assembled capsids, not present in denatured capsid proteins and native but unassembled capsid proteins. The antibody cannot be used for immunoblotting.
Epitope mapping experiments (Wobus et al. 2000) identified four immunoreactive (discontinous) regions. The major reaction was attributed to sequence aa 369 to aa378 of AAV2 capsids. The antibody is also useful for neutralizing experiments.
Wobus, C. E. et al. Monoclonal antibodies against the adeno-associated virus type 2 (AAV-2) capsid: epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection. J. Virol. 74, 9281-93 (2000).
Moskalenko, M. et al. Epitope Mapping of Human Anti-Adeno-Associated Virus Type 2 Neutralizing Antibodies: Implications for Gene Therapy and Virus Structure. Jounal Virol. 74, 1761-1766 (2000).
Limited Use Label License: Research Use Only
Product is exclusively licensed to PROGEN Biotechnik GmbH. The use of these products for the development, manufacturing and sale of secondary products/derivatives which are based on the purchased products and/or which include the purchased product require a royalty based sub-license agreement.
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