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Reactivos e instrumentos para inmunología, biología celular y biología molecular.

Mouse monoclonal antibody to Histone H3

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Referencia CPA9189-30ul

embalaje : 30ul

Contact local distributor :

Teléfono : +1 850 650 7790

Marca : Cohesion Biosciences

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Contact local distributor :

Teléfono : +1 850 650 7790

Western blot analysis of Histone H3 expression in Hela (A), Raw264.7 (B), mouse brain (C), rat brain (D) whole cell lysates. (Predicted band size: 15 kD; Observed band size: 17 kD)
Immunofluorescent analysis of Histone H3 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Immunoprecipitation of Histone H3 from 0.5mg Hela whole cell extract lysate, using Anti-Histone H3 Antibody.

Product Name:	Anti-Histone H3 Antibody
Cat No:	CPA9189
Source:	Mouse
Reactivity:	H, M, R, Yeast
Applications:	WB, IF/IC, IP
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference

Description：Mouse monoclonal antibody to Histone H3

Immunogen：Recombinant protein corresponding to human Histone H3.

Purification：Affinity chromatography

Clonality：Monoclonal

Form：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution：WB (1/2000 - 1/5000), IF/IC (1/100 - 1/500), IP (1/50 - 1/200)

Gene Symbol：HIST1H3A; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; HIST2H3A; HIST2H3C; HIST2H3D; H3F3A; H3F3B

Alternative Names：HIST1H3A; H3FA; HIST1H3B; H3FL; HIST1H3C; H3FC; HIST1H3D; H3FB; HIST1H3E; H3FD; HIST1H3F; H3FI; HIST1H3G; H3FH; HIST1H3H; H3FK; HIST1H3I; H3FF; HIST1H3J; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; HIST2H3A; HIST2H3C; H3F2; H3FM; HIST2H3D; Histone H3.2; Histone H3/m; Histone H3/o; H3F3A; H3.3A; H3F3; PP781; H3F3B; H3.3B; Histone H3.3

Entrez Gene (Human)： 8350; 8351; 8352; 8353; 8354; 8355; 8356; 8357; 8358; 8968;

Entrez Gene (Mouse)： 319152; 15077; 15078;

Entrez Gene (Rat)： 291159; 100361558;

SwissProt (Human)： P68431; Q71DI3; P84243;

SwissProt (Mouse)： P68433; P84228; P84244;

SwissProt (Rat)： Q6LED0; P84245;

Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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