Product Details
Mouse TrueBlot® ULTRA: Anti-Mouse Ig HRP - 18-8817-30
Anti-Mouse IgG HRP, TrueBlot, HRP TrueBlot ULTRA, Peroxidase TrueBlot, TrueBlot for IP/WB, TrueBlot for immunoprecipitation, TrueBlot for western blotting
Rat
Peroxidase (HRP) ULTRA
Monoclonal
IgG
Target Details
Mouse
Mouse TrueBlot® ULTRA Antibody Peroxidase Conjugate was prepared from tissue culture supernatant by Protein G affinity chromatography. Assay by immunoelectrophoresis resulted in a single precipitin arc against Anti-Mouse Serum. Reactivity is observed against native Mouse IgG by both Western blot and ELISA.
Application Details
ELISA, IP, WB
Biochemical Assay, ChIP, IF - View References
Mouse TrueBlot® ULTRA has been tested in ELISA, IP, and Western blot and may also be used for detection in immunoblotting assays that do not employ immunoprecipitation. Mouse IgG TrueBlot® ULTRA is provided as 1000X solution. To achieve best results when detecting mouse IgG1 subtypes, we recommend performing a dot blot or titration to determine the optimal dilution factor for your desired application. All recommended dilutions for listed applications are intended as an initial recommendation, specific conditions for each protein and antibody combination should be specifically optimized by the end user. In order to conserve reagent, we recommend incubating the blots from minigels in sealed bags (removing as much air as possible) with minimal volume (2-3 mLs). If used conservatively at 2.5mls/blot will yield enough reagent for 8 blots. Note that there are three key procedural considerations: 1. Protein A or G beads may be used with the mouse, goat and sheep TrueBlot secondaries, but not with the rabbit TrueBlot secondary. Use of protein A or G beads with the rabbit TrueBlot will result in contaminating bands. 2. Immunoprecipitate should be completely reduced. 3. BLOTTO/Milk should be used as the blocking protein for the immunoblot.
Formulation
1.0 mg/mL
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Proclin is added as an antimicrobial agent.
0.1 mg/ml Bovine Serum Albumin (BSA) - IgG and Protease free, 50% (v/v) Glycerol
Shipping & Handling
Wet Ice
Store at -20°C. This product is guaranteed for 6 months upon receipt, when handled and stored as instructed.
Expiration date is six (6) months from date of receipt.
Mouse IgG TrueBlot, IgG2a, IgG2b, IgG3) over the reduced, SDS-denatured form of IgG. When the immunoprecipitate is fully reduced immediately prior to SDS-gel electrophoresis, reactivity of Mouse IgG TrueBlot kDa heavy chains and the 23 kDa light chains of the immunoprecipitating antibody is minimized thereby eliminating interference by the heavy and light chains of the immunoprecipitating antibody in IP/immunoblot applications. Applications include studies examining post-translational modification (e.g., phosphorylation or acetylation) or protein-protein interactions.
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IP, Co-IP PubMed
Fang Z et al. (2023). Aurora A polyubiquitinates the BRCA1-interacting protein OLA1 to promote centrosome maturation. Cell Rep.
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WB, IB, PCA Jin D et al. (2023). α2δ protein drives opioid">
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Pappas G et al. (2023). MDC1 maintains active elongation complexes of RNA polymerase II. Cell Rep.
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Gong L et al. (2023). AKT Phosphorylates FAM13A and Promotes Its Degradation via CUL4A/DDB1/DCAF1 E3 Complex. Am J Repair Cell Mol Biol.
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IP, Co-IP; WB, IB, PCA PubMed
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Blue SM et al. (2022). Transcriptome-wide identification of RNA-binding protein binding sites using seCLIP-seq. Nat Protoc.
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Alerasool N et al. (2022). Identification and functional characterization of transcriptional activators in human cells. Mol Cel.
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WB, IB, PCA PubMed
Shen JZ et al. (2022). A FBXO7/EYA2-SCFFBXW7 axis promotes AXL-mediated maintenance of mesenchymal and immune evasion phenotypes of cancer cells. Mol Cell.
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WB, IB, PCA PubMed
Liang G et al. (2022). CTNNBL1 restricts HIV-1 replication by suppressing viral DNA integration into the cell genome. Cell Rep.
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WB, IB, PCA PubMed
Cha SJ et al. (2022). Therapeutic modulation of GSTO activity rescues FUS-associated neurotoxicity via deglutathionylation in ALS disease models. Dev Cell.
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WB, IB, PCA PubMed
Lahree A et al. (2022). Active APPL1 sequestration by Plasmodium favors liver-stage development. Cell Rep.
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WB, IB, PCA PubMed
Huang X et al. (2022). A TET1-PSPC1-Neat1 molecular axis modulates PRC2 functions in controlling stem cell bivalency. Cell Rep.
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IP, Co-IP PubMed
Zeng LW et al. (2022). The membrane-associated ubiquitin ligases MARCH2 and MARCH3 target IL-5 receptor alpha to negatively regulate eosinophilic airway inflammation. Cell Mol Immunol.
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WB, IB, PCA PubMed
Volkmar N et al. (2022). Regulation of membrane fluidity by RNF145. EMBO J.
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WB, IB, PCA PubMed
Desjardins P et al. (2022). The WNK1 kinase regulates the stability of transcription factors during wound healing of human corneal epithelial cells. J Cell Physiol.
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WB, IB, PCA PubMed
Namba Y et al. (2022). Maelstrom functions in the production of Siwi-piRISC capable of regulating transposons in Bombyx germ cells. iScience.
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WB, IB, PCA PubMed
Sarodaya N et al. (2022). Deubiquitinase USP19 enhances phenylalanine hydroxylase protein stability and its enzymatic activity. Cell Biol Toxicol.
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WB, IB, PCA PubMed
Saha B et al. (2022). Interactomic analysis reveals a homeostatic role for the HIV restriction factor TRIM5α in mitophagy. Cell Rep.
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WB, IB, PCA PubMed
Banerjee Sle et al. (2022). EPH receptor tyrosine kinases phosphorylate the PAR-3 scaffold protein to modulate downstream signaling networks. Cell Rep.
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WB, IB, PCA PubMed
Kucharski TJ et al. (2022). Small changes in phospho-occupancy at the kinetochore-microtubule interface drive mitotic fidelity. J Cell Biol.
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WB, IB, PCA PubMed
Sarodaya N et al. (2022). Deubiquitinase USP19 extends the residual enzymatic activity of phenylalanine hydroxylase variants. Sci Rep.
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WB, IB, PCA PubMed
Al-Habsi M et al. (2022). Spermidine activates mitochondrial trifunctional protein and improves antitumor immunity in mice. Science.
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WB, IB, PCA PubMed
McCool MA et al. (2022). Human pre-60S assembly factors link rRNA transcription to pre-rRNA processing. RNA.
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WB, IB, PCA PubMed
Watanabe S et al. (2022). Skeletal muscle releases extracellular vesicles with distinct protein and microRNA signatures that function in the muscle microenvironment. PNAS Nexus.
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WB, IB, PCA PubMed
Walker AK et al. (2022). Modification of the SUMO activating enzyme subunit SAE2 directs SUMO isoform bias required for mitotic fidelity. bioRxiv Preprint
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WB, IB, PCA Zhang, Z et al. (2022). Structure of SARS-CoV-2 membrane protein essential for virus assembly. Nature Communications
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WB, IB, PCA PubMed
Gao, Y et al. (2022). Pyrroloquinoline quinone (PQQ) protects mitochondrial function of HEI-OC1 cells under premature senescence. Npj Aging
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IP, Co-IP PubMed
Xiang, Q et al. (2022). STAT and Janus kinase targeting by human herpesvirus 8 interferon regulatory factor in the suppression of type-I interferon signaling. PloS Pathogens
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WB, IB, PCA PubMed
Lankford, C et al. (2022). Identification of HCN1 as a 14-3-3 client. PloS One
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IP, Co-IP PubMed
Gayatri, MB et al. (2022). High glutamine suppresses osteogenesis through mTORC1-mediated inhibition of the mTORC2/AKT-473/RUNX2 axis. Cell Death Discovery
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WB, IB, PCA; IP, Co-IP PubMed
Niewolik, D et al. (2022). Physical ARTEMIS:DNA-PKcs interaction is necessary for V(D)J recombination. Nucleic Acids Research
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WB, IB, PCA PubMed
Matsuda, Y et al. (2022). Phosphorylation of hTERT at threonine 249 is a novel tumor biomarker of aggressive cancer with poor prognosis in multiple organs. The Journal of Pathology
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IP, Co-IP PubMed
Papadopoulos D et al. (2022). MYCN recruits the nuclear exosome complex to RNA polymerase II to prevent transcription-replication conflicts. Mol Cell.
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WB, IB, PCA PubMed
Ambrosi G et al. (2022). Allele-specific endogenous tagging and quantitative analysis of β-catenin in colorectal cancer cells. Elife.
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WB, IB, PCA PubMed
Najafov A et al. (2021). RIPK1 Promotes Energy Sensing by the mTORC1 Pathway. Mol Cell
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WB, IB, PCA PubMed
Kaneya Y et al. (2021). Inhibitor for protein disulfide-isomerase family A member 3 enhances the antiproliferative effect of inhibitor for mechanistic target of rapamycin in liver cancer: An in vitro study on combination treatment with everolimus and 16F16. Oncol Lett.
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WB, IB, PCA PubMed
Alzhanova D et al. (2021). Novel modulators of p53-signaling encoded by unknown genes of emerging viruses. Plos Pathog
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WB, IB, PCA PubMed
Ruthnick D et al. (2021). The N-terminus of Sfi1 and yeast centrin Cdc31 provide the assembly site for a new spindle pole body. J Cell Biol
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WB, IB, PCA PubMed
Yang C et al. (2021). Kidney injury molecule-1 is a potential receptor for SARS-CoV-2. J Mol Cell Biol
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WB, IB, PCA PubMed
Zuliani I et al. (2021). The Dysregulation of OGT/OGA Cycle Mediates Tau and APP Neuropathology in Down Syndrome. Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics
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WB, IB, PCA PubMed
Zuliani I et al. (2021). High-Fat Diet Leads to Reduced Protein O-GlcNAcylation and Mitochondrial Defects Promoting the Development of Alzheimer's Disease Signatures. International Journal of Molecular Sciences
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IP, Co-IP; WB, IB, PCA PubMed
Ali SR et al. (2021). Angiocrine IGFBP3 spatially coordinates IGF signaling during neonatal cardiac regeneration. bioRxiv Preprint
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WB, IB, PCA Rojas-Lopez M et al. (2021). Pattern Recognition Receptor for Bacterial Lipopolysaccharide in the Cytosol of Human Macrophages. bioRxiv Preprint
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WB, IB, PCA Ali S et al. (2021). Angiocrine IGFBP3 spatially coordinates IGF signaling during neonatal cardiac regeneration. bioRxiv Preprint
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WB, IB, PCA Source
Uchida Y et al. (2021). RLTPR Q575E: A novel recurrent gain">
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WB, IB, PCA PubMed
Joshi CS et al. (2021). NRF2 promotes urothelial cell response to bacterial infection by regulating reactive oxygen species and RAB27B expression. Cell Rep.
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IP, Co-IP PubMed
Kumar A et al. (2021). Alanine Catabolism of Paneth Cells Maintains Intestinal Stem Cell Function During Dietary Restriction. Cell Press Preprint
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WB, IB, PCA Source
Sun Y et al. (2021). PARylation prevents the proteasomal degradation of topoisomerase I DNA-protein crosslinks and induces their deubiquitylation. Nat Commun.
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WB, IB, PCA PubMed
Leppek K et al. (2021). VELCRO-IP RNA-seq reveals ribosome expansion segment function in translation genome-wide. Cell Rep.
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WB, IB, PCA PubMed
Wang J et al. (2021). Phase separation of OCT4 controls TAD reorganization to promote cell fate transitions. Cell Stem Cell.
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WB, IB, PCA PubMed
Metzger K et al. (2021). Effects of temperature on proliferation of myoblasts from donor piglets with different thermoregulatory maturities. BMC Mol Cell Biol.
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WB, IB, PCA PubMed
Kim J et al. (2021). Anti-adipocyte differentiation activity and flavonoid content determination by HPLC/UV analysis of tree sprouts. KoreaScience
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WB, IB, PCA Ahn JJ et al. (2021). Protective effects of Camellia sinensis fruit and fruit peels against oxidative DNA damage. KoreaScience
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WB, IB, PCA Jang TW et al. (2021). Anti-Inflammatory Effects of Abeliophyllum distichum Nakai (Cultivar Okhwang 1) Callus through Inhibition of PI3K/Akt, NF-κB, and MAPK Signaling Pathways in Lipopolysaccharide-Induced Macrophages. Processes.
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WB, IB, PCA Source
Matsuda S et al. (2021). Subunit-dependent and independent rules of AMPA receptor trafficking during long-term depression in hippocampal neurons. J Biol Chem.
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WB, IB, PCA PubMed
Nakayama A et al. (2021). Characterisation of a novel KRAS G12C inhibitor ASP2453 that shows potent anti-tumour activity in KRAS G12C-mutated preclinical models. Br J Cancer.
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WB, IB, PCA PubMed
Li X et al. (2021). CUL3 (cullin 3)-mediated ubiquitination and degradation of BECN1 (beclin 1) inhibit autophagy and promote tumor progression. Autophagy.
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WB, IB, PCA PubMed
Kawashima T et al. (2021). Phosphorylation of Collapsin Response Mediator Protein 1 (CRMP1) at Tyrosine 504 residue regulates Semaphorin 3A">
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WB, IB, PCA PubMed
Hazan R et al. (2021). E2F4's cytoplasmic role in multiciliogenesis is mediated via an N-terminal domain that binds two components of the centriole replication machinery, Deup1 and SAS6. Mol Biol Cell.
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WB, IB, PCA PubMed
Piette BL et al. (2021). Comprehensive interactome profiling of the human Hsp70 network highlights functional differentiation of J domains. Mol Cell.
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WB, IB, PCA PubMed
Higa M et al. (2021). TRF2-mediated ORC recruitment underlies telomere stability upon DNA replication stress. Nucleic Acids Res.
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WB, IB, PCA PubMed
Lanzillotta C et al. (2021). Chronic PERK induction promotes Alzheimer-like neuropathology in Down syndrome: Insights for therapeutic intervention. Prog Neurobiol.
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WB, IB, PCA PubMed
Tavernier N et al. (2021). Bora phosphorylation substitutes in trans for T-loop phosphorylation in Aurora A to promote mitotic entry. Nat Commun.
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WB, IB, PCA PubMed
Lankford C et al. (2021). Identification of HCN1 as a 14-3-3 client. bioRxiv Preprint
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WB, IB, PCA Source
Ueda K et al. (2021). MDMX acts as a pervasive preleukemic-to-acute myeloid leukemia transition mechanism. Cancer Cell.
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WB, IB, PCA PubMed
Banerjee SL et al. (2021). Proximity proteomics reveal EPH tyrosine kinase receptors' regulation of cell polarity and identify Par3 scaffold protein as a novel effector of EPH signaling. bioRxiv Preprint
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WB, IB, PCA Source
Iwakawa HO et al. (2021). Ribosome stalling caused by the Argonaute-microRNA-SGS3 complex regulates the production of secondary siRNAs in plants. Cell Rep.
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WB, IB, PCA PubMed
Rojas-Lopez M et al. (2021). Cytosolic Sensor of Bacterial Lipopolysaccharide in Human Macrophages. bioRxiv Preprint
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WB, IB, PCA Source
Toyomoto M et al. (2021). S1PR3-biased agonist ALESIA targets cancer metabolism and promotes glucose starvation. Cell Chem Biol.
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WB, IB, PCA PubMed
Lohani S et al. (2021). A novel role of PRL in regulating epithelial cell density by inducing apoptosis at confluence. J Cell Sci.
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WB, IB, PCA PubMed
Moore J et al. (2021). Random errors in protein synthesis activate an age-dependent program of muscle atrophy in mice. Commun Biol.
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Alzhanova D et al. (2021). The ORF45 Protein of Kaposi Sarcoma-Associated Herpesvirus Is an Inhibitor of p53 Signaling during Viral Reactivation. J Virol.
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WB, IB, PCA PubMed
Kucharski TJ et al. (2021). Chromosome segregation fidelity is controlled by small changes in phospho-occupancy at the kinetochore-microtubule interface. bioRxiv Preprint
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WB, IB, PCA Source
Ukaji T et al. (2021). Novel knock">
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WB, IB, PCA PubMed
He X et al. (2021). Tumor-initiating stem cell shapes its microenvironment into an immunosuppressive barrier and pro-tumorigenic niche. Cell Rep.
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Hitachi K et al. (2021). Myoparr-associated and-independent multiple roles of heterogeneous nuclear ribonucleoprotein k during skeletal muscle cell differentiation. Int J Mol Sci.
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WB, IB, PCA PubMed
Miyagi E et al. (2020). Inhibition of Vif-Mediated Degradation of APOBEC3G through Competitive Binding of Core-Binding Factor Beta. J Virol
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WB, IB, PCA PubMed
Pereira C et al. (2020). Nuclear Accumulation of LAP1: TRF2 Complex during DNA Damage Response Uncovers a Novel Role for LAP1. Cells
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WB, IB, PCA PubMed
Lewis A et al. (2020). A Family of Argonaute-Interacting Proteins Gates Nuclear RNAi. Mol Cell
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WB, IB, PCA PubMed
Tsukiyama T et al. (2020). A phospho-switch controls RNF43-mediated degradation of Wnt receptors to suppress tumorigenesis. Nat Commun
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Fuentes-Iglesias A et al. (2020). An Optimized Immunoprecipitation Protocol for Assessing Protein-RNA Interactions In Vitro. STAR Protoc.
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WB, IB, PCA PubMed
Nilsson J et al. (2020). NKT cells promote both type 1 and type 2 inflammatory responses in a mouse model of liver fibrosis. Sci Rep.
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WB, IB, PCA PubMed
Lear et al. (2020). Kelch-like protein 42 is a pro-fibrotic ubiquitin E3 ligase involved in in systemic sclerosis. Journal of Biological Chemistry
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IP, Co-IP; WB, IB, PCA PubMed
Lear et al. (2020). Kelch-like protein 42 is a pro-fibrotic ubiquitin E3 ligase involved in in systemic sclerosis. Journal of Biological Chemistry
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WB, IB, PCA PubMed
Jang, T. et al. (2020). Protective and inhibitory effects of acteoside from Abeliophyllum distichum Nakai against oxidative DNA damage. Molecular Medicine Reports
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WB, IB, PCA PubMed
Zhou Q, Cheng C, Wei Y, et al. (2020). USP15 potentiates NF-κB activation by differentially stabilizing TAB2 and TAB3. FEBS J.
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WB, IB, PCA PubMed
Kwan SY, Au-Yeung CL, Yeung TL, et al. (2020). Ubiquitin Carboxyl-Terminal Hydrolase L1 (UCHL1) Promotes Uterine Serous Cancer Cell Proliferation and Cell Cycle Progression. Cancers (Basel).
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IP, Co-IP PubMed
Li Q, Sun Y, Jarugumilli GK, et al. (2020). Lats1/2 Sustain Intestinal Stem Cells and Wnt Activation through TEAD-Dependent and Independent Transcription. Cell Stem Cell.
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Undefined PubMed
Zhou H, Blevins MA, Hsu JY, et al. (2020). Identification of a Small-Molecule Inhibitor That Disrupts the SIX1/EYA2 Complex, EMT, and Metastasis. Cancer Res.
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WB, IB, PCA PubMed
Bacon CW, Challa A, Hyder U, et al. (2020). KAP1 Is a Chromatin Reader that Couples Steps of RNA Polymerase II Transcription to Sustain Oncogenic Programs. Mol Cell.
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IP, Co-IP PubMed
Rosàs-Canyelles E, Modzelewski AJ, Geldert A, He L, Herr AE. (2020). Assessing heterogeneity among single embryos and single blastomeres using open microfluidic design. Sci Adv.
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WB, IB, PCA PubMed
Jang TW, Choi JS, Park JH. (2020). Protective and inhibitory effects of acteoside from Abeliophyllum distichum Nakai against oxidative DNA damage. Mol Med Rep.
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WB, IB, PCA PubMed
Wuerth JD, Habjan M, Kainulainen M, et al. (2020). eIF2B as a Target for Viral Evasion of PKR-Mediated Translation Inhibition. mBio.
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WB, IB, PCA PubMed
Wang Q, Zhang T, Chang X, et al. (2020). Targeting Opsin4/Melanopsin with a Novel Small Molecule Suppresses PKC/RAF/MEK/ERK Signaling and Inhibits Lung Adenocarcinoma Progression. Mol Cancer Res.
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WB, IB, PCA PubMed
Wheeler EC, Vu AQ, Einstein JM, et al. (2020). Pooled CRISPR screens with imaging on microraft arrays reveals stress granule-regulatory factors. Nat Methods.
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WB, IB, PCA PubMed
Johnson JS, De Veaux N, Rives AW, et al. (2020). A Comprehensive Map of the Monocyte-Derived Dendritic Cell Transcriptional Network Engaged upon Innate Sensing of HIV. Cell Rep.
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WB, IB, PCA PubMed
Bie AS, Cömert C, Körner R, et al. (2020). An inventory of interactors of the human HSP60/HSP10 chaperonin in the mitochondrial matrix space. Cell Stress Chaperones.
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WB, IB, PCA PubMed
Brekk OR, Korecka JA, Crapart CC, et al. (2020). Upregulating β-hexosaminidase activity in rodents prevents α-synuclein lipid associations and protects dopaminergic neurons from α-synuclein-mediated neurotoxicity. Acta Neuropathol Commun.
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WB, IB, PCA PubMed
Lin VJT, Hu J, Zolekar A, Yan LJ, Wang YC. (2020). Urine Sample-Derived Cerebral Organoids Suitable for Studying Neurodevelopment and Pharmacological Responses. Front Cell Dev Biol.
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IP, Co-IP PubMed
Msaouel P et al. (2020). Comprehensive molecular characterization identifies distinct genomic and immune hallmarks of renal medullary carcinoma. Cancer Cell.
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WB, IB, PCA PubMed
Kubo Y et al. (2020). Periostin and tenascin-C interaction promotes angiogenesis in ischemic proliferative retinopathy. Sci Rep.
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IP, Co-IP; WB, IB, PCA PubMed
Huang T et al. (2020). Fbxo7 and Pink1 play a reciprocal role in regulating their protein levels. Aging (Albany NY).
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WB, IB, PCA PubMed
Joshi CS et al. (2020). Oxidative Stress Culminates in Intracellular Bacteria Expulsion Via NRF2/KEAP1 Induction of RAB27B. Cell Press Preprint
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WB, IB, PCA Source
Liu Y et al. (2020). WSTF acetylation by MOF promotes WSTF activities and oncogenic functions. Oncogene.
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Undefined PubMed
Leppek K et al. (2020). Gene-and Species-Specific hox mRNA translation by ribosome expansion segments. Mol Cell.
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WB, IB, PCA PubMed
Ko JH et al. (2020). TREX2 Exonuclease Causes Spontaneous Mutations and Stress-Induced Replication Fork Defects in Cells Expressing RAD51K133A. Cell Rep.
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WB, IB, PCA PubMed
Dolicka D et al. (2020). Tristetraprolin promotes hepatic inflammation and tumor initiation but restrains cancer progression to malignancy. Cell Mol Gastroenterol Hepatol.
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WB, IB, PCA PubMed
Tian X et al. (2020). Inhibiting calpain 1 and 2 in cyclin G associated kinase">
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WB, IB, PCA PubMed
Karam N et al. (2019). Bone-Specific Overexpression of PITX1 Induces Senile Osteoporosis in Mice Through Deficient Self-Renewal of Mesenchymal Progenitors and Wnt Pathway Inhibition. Sci Rep.
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WB, IB, PCA PubMed
Mehto et al. (2019). The Crohn's Disease Risk Factor IRGM Limits NLRP3 Inflammasome Activation by Impeding Its Assembly and by Mediating Its Selective Autophagy. Molecular Cell
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WB, IB, PCA PubMed
Mehto et al. (2019). The Crohn's Disease Risk Factor IRGM Limits NLRP3 Inflammasome Activation by Impeding Its Assembly and by Mediating Its Selective Autophagy. Molecular Cell
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IP, Co-IP PubMed
Graves-Deal et al. (2019). Broad-spectrum receptor tyrosine kinase inhibitors overcome de novo and acquired modes of resistance to EGFR-targeted therapies in colorectal cancer. Oncotarget
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WB, IB, PCA PubMed
Zhao et al. (2019). Microbial recognition by GEF-H1 controls IKKε mediated activation of IRF5. Nature Communications
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IP, Co-IP PubMed
Islam H, Kobayashi M, Honjo T. (2019). Apurinic/apyrimidinic endonuclease 1 (APE1) is dispensable for activation-induced cytidine deaminase (AID)-dependent somatic hypermutation in the immunoglobulin gene. Int Immunol.
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WB, IB, PCA PubMed
Rueda EM, Hall BM, Hill MC, et al. (2019). The Hippo Pathway Blocks Mammalian Retinal Müller Glial Cell Reprogramming. Cell Rep.
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IP, Co-IP PubMed
Crotti A, Sait HR, McAvoy KM, et al. (2019). BIN1 favors the spreading of Tau via extracellular vesicles. Sci Rep.
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WB, IB, PCA PubMed
Crotti A, Sait HR, McAvoy KM, et al. (2019). BIN1 favors the spreading of Tau via extracellular vesicles. Sci Rep.
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WB, IB, PCA; IP, Co-IP PubMed
Meyer SN, Scuoppo C, Vlasevska S, et al. (2019). Unique and Shared Epigenetic Programs of the CREBBP and EP300 Acetyltransferases in Germinal Center B Cells Reveal Targetable Dependencies in Lymphoma. Immunity.
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WB, IB, PCA PubMed
Santos M, Morais S, Pereira C, Sequeiros J, Alonso I. (2019). Parkin truncating variants result in a loss-of-function phenotype. Sci Rep.
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WB, IB, PCA; IP, Co-IP PubMed
Li H, Li Q, Dang K, et al. (2019). YAP/TAZ Activation Drives Uveal Melanoma Initiation and Progression. Cell Rep.
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WB, IB, PCA PubMed
Andersen L, Gülich AF, Alteneder M, et al. (2019). The Transcription Factor MAZR/PATZ1 Regulates the Development of FOXP3+ Regulatory T Cells. Cell Rep.
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WB, IB, PCA PubMed
Dick J.H. van den Boomen, Agata Sienkiewicz, Ilana Berlin, Marlieke L.M. Jongsma, Daphne M. van Elsl (2019). A trimeric Rab7 GEF controls NPC1-dependent lysosomal cholesterol export. Nat Commun
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IF, Confocal Microscopy PubMed
Yang-Hartwich Y, Tedja R, Roberts CM, et al. (2019). p53-Pirh2 Complex Promotes Twist1 Degradation and Inhibits EMT. Mol Cancer Res.
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WB, IB, PCA PubMed
Tedja R, Roberts CM, Alvero AB, et al. (2019). Protein kinase Cα-mediated phosphorylation of Twist1 at Ser-144 prevents Twist1 ubiquitination and stabilizes it. J Biol Chem.
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WB, IB, PCA PubMed
Najafov A, Mookhtiar AK, Luu HS, et al. (2019). TAM Kinases Promote Necroptosis by Regulating Oligomerization of MLKL. Mol Cell.
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Le-Bel G, Cortez Ghio S, Guérin LP, Bisson F, Germain L, Guérin SL. (2019). Irradiated Human Fibroblasts as a Substitute Feeder Layer to Irradiated Mouse 3T3 for the Culture of Human Corneal Epithelial Cells: Impact on the Stability of the Transcription Factors Sp1 and NFI. Int J Mol Sci.
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Boonying W, Joselin A, Huang E, et al. (2019). Pink1 regulates FKBP5 interaction with AKT/PHLPP and protects neurons from neurotoxin stress induced by MPP. J Neurochem.
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Paoletti A, Allouch A, Caillet M, et al. (2019). HIV-1 Envelope Overcomes NLRP3-Mediated Inhibition of F-Actin Polymerization for Viral Entry. Cell Rep.
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Kumanishi S, Yamanegi K, Nishiura H, et al. (2019). Epigenetic modulators hydralazine and sodium valproate act synergistically in VEGI-mediated anti-angiogenesis and VEGF interference in human osteosarcoma and vascular endothelial cells. Int J Oncol.
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Marcar L, Bardhan K, Gheorghiu L, et al. (2019). Acquired Resistance of EGFR-Mutated Lung Cancer to Tyrosine Kinase Inhibitor Treatment Promotes PARP Inhibitor Sensitivity. Cell Rep.
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Pattschull G, Walz S, Gründl M, et al. (2019). The Myb-MuvB Complex Is Required for YAP-Dependent Transcription of Mitotic Genes. Cell Rep.
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Shimoda T et al. (2019). Expression of protein disulfide isomerase A3 and its clinicopathological association in gastric cancer. Oncol Rep.
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Hwang JY et al. (2019). Dual Sensing of Physiologic pH and Calcium by EFCAB9 Regulates Sperm Motility. Cell.
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Liu et al. (2018). Cand1-Mediated Adaptive Exchange Mechanism Enables Variation in F-Box Protein Expression. Molecular Cell
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Pasquin et al. (2018). Effect of human very low-density lipoproteins on cardiotrophin-like cytokine factor 1 (CLCF1) activity. Scientific Reports
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Guarnieri et al. (2018). The miR-106b-25 cluster mediates breast tumor initiation through activation of NOTCH1 via direct repression of NEDD4L. Oncogene
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Park et al. (2018). ATP depletion during mitotic arrest induces mitotic slippage and APC/CCdh1-dependent cyclin B1 degradation. Experimental & Molecular Medicine
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Mitchell et al. (2018). IL1RAP potentiates multiple oncogenic signaling pathways in AML. Journal of Experimental Medicine
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Brekk et al. (2018). Lipid-dependent deposition of alpha-synuclein and Tau on neuronal Secretogranin II-positive vesicular membranes with age. Scientific Reports
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Jeon et al. (2018). PDI Knockdown Inhibits Seizure Activity in Acute Seizure and Chronic Epilepsy Rat Models via S-Nitrosylation-Independent Thiolation on NMDA Receptor. Frontiers in Cellular Neuroscience
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Menzies et al. (2018). The sterol-responsive RNF145 E3 ubiquitin ligase mediates the degradation of HMG-CoA reductase together with gp78 and Hrd1. Elife
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IP, Co-IP PubMed
D'Souza et al. (2018). Gankyrin Promotes Tumor-Suppressor Protein Degradation to Drive Hepatocyte Proliferation. Cellular and Molecular Gastroenterology and Hepatology
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WB, IB, PCA PubMed
Kistler et al. (2018). Phase transitioned nuclear Oskar promotes cell division of Drosophila primordial germ cells. Elife
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Ikeuchi et al. (2018). Extracellular Protein Fibulin-7 and Its C-Terminal Fragment Have In Vivo Antiangiogenic Activity. Scientific Reports
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Kumar A, Giri S, Shaha C. (2018). Sestrin2 facilitates glutamine-dependent transcription of PGC-1α and survival of liver cancer cells under glucose limitation. FEBS J.
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Wang Z, Yang B, Zhang M, et al. (2018). lncRNA Epigenetic Landscape Analysis Identifies EPIC1 as an Oncogenic lncRNA that Interacts with MYC and Promotes Cell-Cycle Progression in Cancer. Cancer Cell.
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WB, IB, PCA PubMed
Dai L, Zhao T, Bisteau X, et al. (2018). Modulation of Protein-Interaction States through the Cell Cycle. Cell.
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IP, Co-IP PubMed
Choi SW, Park HH, Kim S, et al. (2018). PELI1 Selectively Targets Kinase-Active RIP3 for Ubiquitylation-Dependent Proteasomal Degradation. Mol Cell.
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Ghezraoui H, Oliveira C, Becker JR, et al. (2018). 53BP1 cooperation with the REV7-shieldin complex underpins DNA structure-specific NHEJ. Nature.
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Brescia P, Schneider C, Holmes AB, et al. (2018). MEF2B Instructs Germinal Center Development and Acts as an Oncogene in B Cell Lymphomagenesis. Cancer Cell.
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Hayashi K, Taura M, Iwasaki A. (2018). The interaction between IKKα and LC3 promotes type I interferon production through the TLR9-containing LAPosome. Sci Signal.
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Yao B, Li Y, Wang Z, et al. (2018). Active N6-Methyladenine Demethylation by DMAD Regulates Gene Expression by Coordinating with Polycomb Protein in Neurons. Mol Cell.
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Kitamura K, Que L, Shimadu M, et al. (2018). Flap endonuclease 1 is involved in cccDNA formation in the hepatitis B virus. PLoS Pathog.
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Hou W, Cruz-Cosme R, Wen F, et al. (2018). Expression of Human Cytomegalovirus IE1 Leads to Accumulation of Mono-SUMOylated PML That Is Protected from Degradation by Herpes Simplex Virus 1 ICP0. J Virol.
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Lahaye X, Gentili M, Silvin A, et al. (2018). NONO Detects the Nuclear HIV Capsid to Promote cGAS-Mediated Innate Immune Activation. Cell. 2018;175(2):488-501.e22. doi:10.1016/j.cell.2018.08.062 Cell.
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Monzón-Casanova E, Screen M, Díaz-Muñoz MD, et al. (2018). The RNA-binding protein PTBP1 is necessary for B cell selection in germinal centers. Nat Immunol.
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Maeda T, Suetake H, Odaka T, Miyadai T. (2018). Original Ligand for LTβR Is LIGHT: Insight into Evolution of the LT/LTβR System. J Immunol.
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Lampert F, Stafa D, Goga A, et al. (2018). The multi-subunit GID/CTLH E3 ubiquitin ligase promotes cell proliferation and targets the transcription factor Hbp1 for degradation. Elife.
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Garrido F, Pacheco M, Vargas-Martínez R, et al. (2018). Identification of hepatic protein-protein interaction targets for betaine homocysteine S-methyltransferase. PLoS One.
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Min Y, Kim MJ, Lee S, Chun E, Lee KY. (2018). Inhibition of TRAF6 ubiquitin-ligase activity by PRDX1 leads to inhibition of NFKB activation and autophagy activation. Autophagy.
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Seidi A, Muellner-Wong LS, Rajendran E, et al. (2018). Elucidating the mitochondrial proteome of Toxoplasma gondii reveals the presence of a divergent cytochrome c oxidase. Elife.
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Mori M, Hazan R, Danielian PS, et al. (2018). Cytoplasmic E2f4 forms organizing centres for initiation of centriole amplification during multiciliogenesis. Nat Commun.
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Zhang et al. (2017). Protein Phosphotyrosine Phosphatase 1B (PTP1B) in Calpain-dependent Feedback Regulation of Vascular Endothelial Growth Factor Receptor (VEGFR2) in Endothelial Cells: IMPLICATIONS IN VEGF-DEPENDENT ANGIOGENESIS AND DIABETIC WOUND HEALING. Journal of Biological Chemistry
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Rivera-Serrano et al. (2017). A Cytoplasmic RNA Virus Alters the Function of the Cell Splicing Protein SRSF2. Journal of Virology
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Mansilla et al. (2017). Interference of the complex between NCS-1 and Ric8a with phenothiazines regulates synaptic function and is an approach for fragile X syndrome. Proc. Natl. Acad. Sci. U.S.A
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Nounamo et al. (2017). An interaction domain in human SAMD9 is essential for myxoma virus host-range determinant M062 antagonism of host anti-viral function. Virology
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Zhai et al. (2017). NLRP1 promotes tumor growth by enhancing inflammasome activation and suppressing apoptosis in metastatic melanoma. Oncogene
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Bourens et al. (2017). Human mitochondrial cytochrome c oxidase assembly factor COX18 acts transiently as a membrane insertase within the subunit 2 maturation module. Journal of Biological Chemistry
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Hu et al. (2017). An FAK-YAP-mTOR Signaling Axis Regulates Stem Cell-Based Tissue Renewal in Mice. Cell Stem Cell
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IF, Confocal Microscopy; WB, IB, PCA PubMed
Lewis et al. (2017). RNA Binding Protein CUGBP1 Inhibits Liver Cancer in a Phosphorylation-Dependent Manner. Molecular and Cellular Biology
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Bononi et al. (2017). BAP1 regulates IP3R3-mediated Ca2+ flux to mitochondria suppressing cell transformation. Nature
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Mori et al. (2017). Cytoplasmic E2f4 forms organizing centres for initiation of centriole amplification during multiciliogenesis. Nature Communications
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Joachim et al. (2017). Centriolar Satellites Control GABARAP Ubiquitination and GABARAP-Mediated Autophagy. Current Biology
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Miller et al. (2017). A Brucella Type IV Effector Targets the COG Tethering Complex to Remodel Host Secretory Traffic and Promote Intracellular Replication. Cell Host Microbe
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Miller et al. (2017). A Brucella Type IV Effector Targets the COG Tethering Complex to Remodel Host Secretory Traffic and Promote Intracellular Replication. Cell Host Microbe
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IF, Confocal Microscopy PubMed
Weng et al. (2017). POPX2 phosphatase regulates apoptosis through the TAK1-IKK-NF-κB pathway. Cell Death & Disease
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Hiyama et al. (2017). Blue light and CO2 signals converge to regulate light-induced stomatal opening. Nature Communications
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Lehmann et al. (2017). Mechanistic Insights into Autoinhibition of the Oncogenic Chromatin Remodeler ALC1. Molecular Cell
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Lu et al. (2017). Cell cycle-dependent phosphorylation regulates RECQL4 pathway choice and ubiquitination in DNA double-strand break repair. Nature Communications
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Seong et al. (2017). Zinc finger protein ZPR9 functions as an activator of AMPK-related serine/threonine kinase MPK38/MELK involved in ASK1/TGF-β/p53 signaling pathways. Scientific Reports
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Kashihara et al. (2017). Angiotensin II activates CaV1.2 Ca2+channels through β-arrestin2 and casein kinase 2 in mouse immature cardiomyocytes. The Journal of Physiology
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Torregrosa-Muñumer R, Forslund JME, Goffart S, et al. (2017). PrimPol is required for replication reinitiation after mtDNA damage. Proc Natl Acad Sci U S A.
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Reitsma JM, Liu X, Reichermeier KM, et al. (2017). Composition and Regulation of the Cellular Repertoire of SCF Ubiquitin Ligases. Cell.
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Saud K, Cánovas J, Lopez CI, et al. (2017). SFPQ associates to LSD1 and regulates the migration of newborn pyramidal neurons in the developing cerebral cortex. Int J Dev Neurosci.
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Simsek D, Tiu GC, Flynn RA, et al. (2017). The Mammalian Ribo-interactome Reveals Ribosome Functional Diversity and Heterogeneity. Cell.
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Iwahori S, Umaña AC, VanDeusen HR, Kalejta RF. (2017). Human cytomegalovirus-encoded viral cyclin-dependent kinase (v-CDK) UL97 phosphorylates and inactivates the retinoblastoma protein-related p107 and p130 proteins. J Biol Chem.
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Iwahori S, Kalejta RF. (2017). Phosphorylation of transcriptional regulators in the retinoblastoma protein pathway by UL97, the viral cyclin-dependent kinase encoded by human cytomegalovirus. Virology.
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Perez KJ, Martínez FP, Cosme-Cruz R, Perez-Crespo NM, Tang Q. Correction for Perez et al., (2017). A Short cis-Acting Motif in the M112-113 Promoter Region Is Essential for IE3 To Activate M112-113 Gene Expression and Is Important for Murine Cytomegalovirus Replication. J Virol.
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Roncero et al. (2016). Contribution of JAK2 mutations to T-cell lymphoblastic lymphoma development. Leukemia
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Vijayalingam et al. (2016). The Cellular Protein Complex Associated with a Transforming Region of E1A Contains c-MYC. Journal of Virology
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Vijayalingam et al. (2016). The Cellular Protein Complex Associated with a Transforming Region of E1A Contains c-MYC. Journal of Virology
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Suzuki et al. (2016). Characterization of RyDEN (C19orf66) as an Interferon-Stimulated Cellular Inhibitor against Dengue Virus Replication. PLOS Pathogens
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Narusaka et al. (2016). Leucine zipper motif in RRS1 is crucial for the regulation of Arabidopsis dual resistance protein complex RPS4/RRS1. Scientific Reports
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Kenific et al. (2016). NBR1 enables autophagy-dependent focal adhesion turnover. Journal of Cell Biology
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Saito et al. (2016). Oral administration of Aloe vera gel powder prevents UVB-induced decrease in skin elasticity via suppression of overexpression of MMPs in hairless mice. Bioscience, Biotechnology, and Biochemistry
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Zhang et al. (2016). Direct Demonstration That Loop1 of Scap Binds to Loop7: A CRUCIAL EVENT IN CHOLESTEROL HOMEOSTASIS. Journal of Biological Chemistry
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Akiyama et al. (2016). Cyclic Nucleotide Control of Microtubule Dynamics for Axon Guidance. The Journal of Neuroscience
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Granados-Durán et al. (2016). Complement system activation contributes to the ependymal damage induced by microbial neuraminidase. Journal of Neuroinflammation
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Aly et al. (2016). RNA Exosome Complex Regulates Stability of the Hepatitis B Virus X-mRNA Transcript in a Non-stop-mediated (NSD) RNA Quality Control Mechanism. Journal of Biological Chemistry
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Luo et al. (2016). SMG7 is a critical regulator of p53 stability and function in DNA damage stress response. Cell Discovery
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Min et al. (2016). Cereblon negatively regulates TLR4 signaling through the attenuation of ubiquitination of TRAF6. Cell Death & Disease
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McCormick et al. (2016). Suppressor of Cytokine Signaling (SOCS)1 Regulates Interleukin-4 (IL-4)-activated Insulin Receptor Substrate (IRS)-2 Tyrosine Phosphorylation in Monocytes and Macrophages via the Proteasome. Journal of Biological Chemistry
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IP, Co-IP PubMed
Jungas et al. (2016). Eph-mediated tyrosine phosphorylation of citron kinase controls abscission. Journal of Cell Biology
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IP, Co-IP; WB, IB, PCA PubMed
Jeganathan et al. (2016). Rac1-mediated cytoskeleton rearrangements induced by intersectin-1s deficiency promotes lung cancer cell proliferation, migration and metastasis. Molecular Cancer
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IP, Co-IP; WB, IB, PCA PubMed
Urbanus et al. (2016). Diverse mechanisms of metaeffector activity in an intracellular bacterial pathogen, Legionella pneumophila. Molecular Systems Biology
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Araki M, Yang Y, Masubuchi N, et al. (2016). Activation of the thrombopoietin receptor by mutant calreticulin in CALR-mutant myeloproliferative neoplasms. Blood.
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Banerjee R, Russo N, Liu M, et al. : (2016). TRIP13 promotes error-prone nonhomologous end joining and induces chemoresistance in head and neck cancer. Nat Commun.
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Masser DR, Clark NW, Van Remmen H, Freeman WM. (2016). Loss of the antioxidant enzyme CuZnSOD (Sod1) mimics an age-related increase in absolute mitochondrial DNA copy number in the skeletal muscle. Age (Dordr).
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Pérez C, Pérez-Zúñiga FJ, Garrido F, Reytor E, Portillo F, Pajares MA. (2016). The Oncogene PDRG1 Is an Interaction Target of Methionine Adenosyltransferases. PLoS One.
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Rohnalter V et al. (2015). A multi-stage process including transient polyploidization and EMT precedes the emergence of chemoresistent ovarian carcinoma cells with a dedifferentiated and pro-inflammatory secretory phenotype. Oncotarget
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St-Denis et al. (2015). Myotubularin-related proteins 3 and 4 interact with polo-like kinase 1 and centrosomal protein of 55 kDa to ensure proper abscission. Molecular & Cellular Proteomics
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IP, Co-IP PubMed
Li et al. (2015). A monoclonal antibody that tracks endospore formation in the microsporidium Nosema bombycis. PLOS One
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Gustin et al. (2015). Ebola Virus Glycoprotein Promotes Enhanced Viral Egress by Preventing Ebola VP40 From Associating With the Host Restriction Factor BST2/Tetherin. The Journal of Infectious Diseases
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IP, Co-IP; WB, IB, PCA PubMed
Dagvadorj et al. (2015). Lipopolysaccharide Induces Alveolar Macrophage Necrosis via CD14 and the P2X7 Receptor Leading to Interleukin-1α Release. Immunity
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Greenfeld et al. (2015). TRAF1 Coordinates Polyubiquitin Signaling to Enhance Epstein-Barr Virus LMP1-Mediated Growth and Survival Pathway Activation. PLOS Pathogens
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Ibeawuchi et al. (2015). Hypertension-causing Mutations in Cullin3 Protein Impair RhoA Protein Ubiquitination and Augment the Association with Substrate Adaptors. Journal of Biological Chemistry
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Vuong et al. (2015). Differential Effects of Hepatocyte Nuclear Factor 4α Isoforms on Tumor Growth and T-Cell Factor 4/AP-1 Interactions in Human Colorectal Cancer Cells. Molecular and Cellular Biology
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ChIP; WB, IB, PCA PubMed
Namba et al. (2015). Loss of p53 enhances the function of the endoplasmic reticulum through activation of the IRE1α/XBP1 pathway. Oncotarget
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Sakaguchi et al. (2015). MAZR and Runx Factors Synergistically Repress ThPOK during CD8+ T Cell Lineage Development. Journal of Immunology
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Sakaguchi et al. (2015). MAZR and Runx Factors Synergistically Repress ThPOK during CD8+ T Cell Lineage Development. Journal of Immunology
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Lin et al. (2015). Expression of human carcinoembryonic antigen-related cell adhesion molecule 6 and alveolar progenitor cells in normal and injured lungs of transgenic mice. Physiological Reports
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Liang G, Liu G, Kitamura K, et al. (2015). TGF-β suppression of HBV RNA through AID-dependent recruitment of an RNA exosome complex. PLoS Pathog.
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Li H, Mohamed AA, Sharad S, et al. (2015). Silencing of PMEPA1 accelerates the growth of prostate cancer cells through AR, NEDD4 and PTEN. Oncotarget.
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Iwahori S, Hakki M, Chou S, Kalejta RF. (2015). Molecular Determinants for the Inactivation of the Retinoblastoma Tumor Suppressor by the Viral Cyclin-dependent Kinase UL97. J Biol Chem.
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Nishimura T. (2015). Role of extracellular matrix in development of skeletal muscle and postmortem aging of meat. Meat Sci.
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Xu J, Eriksson SE, Cebula M, et al. (2015). The conserved Trp114 residue of thioredoxin reductase 1 has a redox sensor-like function triggering oligomerization and crosslinking upon oxidative stress related to cell death. Cell Death Dis.
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Yousif AS et al. (2014). Differential regulation of S-region hypermutation and class switch recombination by noncanonical functions of uracil DNA glycosylase. Proc Natl Acad Sci USA
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ChIP; IP, Co-IP PubMed
Puglisi R, Maccari I, Pipolo S, Mangia F, Boitani C. (2014). The nuclear form of glutathione peroxidase 4 colocalizes and directly interacts with protamines in the nuclear matrix during mouse sperm chromatin assembly. Spermatogenesis.
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Jerman S, Ward HH, Lee R, et al. (2014). OFD1 and flotillins are integral components of a ciliary signaling protein complex organized by polycystins in renal epithelia and odontoblasts. PLoS One.
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Petry FR, Pelletier J, Bretteville A, et al. (2014). Specificity of anti-tau antibodies when analyzing mice models of Alzheimer's disease: problems and solutions. PLoS One.
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Liaunardy-Jopeace A, Bryant CE, Gay NJ. (2014). The COP II adaptor protein TMED7 is required to initiate and mediate the delivery of TLR4 to the plasma membrane. Sci Signal.
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Gong H et al. (2013). Lipoprotein lipase (LPL) is associated with neurite pathology and its levels are markedly reduced in the dentate gyrus of Alzheimer's disease brains. The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
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Demir K, Kirsch N, Beretta CA, et al. (2013). RAB8B is required for activity and caveolar endocytosis of LRP6. Cell Rep.
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Stuckenholz C, Lu L, Thakur PC, Choi TY, Shin D, Bahary N. (2013). Sfrp5 modulates both Wnt and BMP signaling and regulates gastrointestinal organogenesis [corrected] in the zebrafish, Danio rerio. PLoS One.
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Ryan AS, Li G, Blumenthal JB, Ortmeyer HK. (2013). Aerobic exercise + weight loss decreases skeletal muscle myostatin expression and improves insulin sensitivity in older adults. Obesity (Silver Spring).
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Murata H, Sakaguchi M, Kataoka K, Huh NH. (2013). SARM1 and TRAF6 bind to and stabilize PINK1 on depolarized mitochondria. Mol Biol Cell.
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Martínez FP, Tang Q. (2012). Leucine zipper domain is required for Kaposi sarcoma-associated herpesvirus (KSHV) K-bZIP protein to interact with histone deacetylase and is important for KSHV replication. J Biol Chem.
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Bonaterra GA, Zügel S, Thogersen J, et al. (2012). Growth differentiation factor-15 deficiency inhibits atherosclerosis progression by regulating interleukin-6-dependent inflammatory response to vascular injury. J Am Heart Assoc.
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Cosme-Cruz R, Martínez FP, Perez KJ, Tang Q. (2011). H2B homology region of major immediate-early protein 1 is essential for murine cytomegalovirus to disrupt nuclear domain 10, but is not important for viral replication in cell culture. J Gen Virol.
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Wang J, Zhang Y, Lu C, Lei L, Yu P, Zhong G. (2010). A genome-wide profiling of the humoral immune response to Chlamydia trachomatis infection reveals vaccine candidate antigens expressed in humans. J Immunol.
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Kim S, Huang W, Mottillo EP, et al. (2010). Posttranslational regulation of membrane type 1-matrix metalloproteinase (MT1-MMP) in mouse PTEN null prostate cancer cells: Enhanced surface expression and differential O-glycosylation of MT1-MMP. Biochim Biophys Acta.
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Prast-Nielsen S, Cebula M, Pader I, Arnér ES. (2010). Noble metal targeting of thioredoxin reductase--covalent complexes with thioredoxin and thioredoxin-related protein of 14 kDa triggered by cisplatin. Free Radic Biol Med.
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What is Rockland; product line?
Rockland; product line is designed to solve common experimental problems when performing immunoprecipitation/co-immunoprecipitation experiments and Western blots of immunoprecipitated samples (IP; monoclonal secondary antibodies and TrueBlot® IP beads.
What is the advantage of TrueBlot® secondaries over regular secondary antibodies?
TrueBlot® antibodies are specific to the whole IgG molecule and will not bind heavy or light chains. This is useful for binding target proteins with an expected MW near 25 kDa (light chains) and 50 kDa (heavy chains).
Why does it appear that the TrueBlot® antibody is binding heavy/light chains in my sample?
The sample must be fully reduced to eliminate cross-reactivity with heavy and light chains. Any reactivity to heavy and light chains could be due to incomplete reduction of your sample. Please be sure to optimize reducing conditions for your sample type.
How can I ensure my samples are fully reduced?
Samples can be fully reduced by heating at 90-100°C for 10 minutes in sample buffer containing reducing agent (for example, DTT to a final concentration of 50 mM or add β-Mercaptoethanol to a final concentration of 2% (v/v)).
With which species/isotypes are TrueBlot® secondary antibodies reactive?
TrueBlot® secondary antibodies are reactive with the IgG of their respective species. For example, if using a primary antibody raised in mouse (mouse host), use TrueBlot® anti-mouse Ig secondary antibodies for detection.
Do TrueBlot® secondary antibodies detect IgM?
TrueBlot® secondary antibodies have not been shown to detect IgM.
Do I need to preclear my lysate before the immunoprecipitation step?
Samples that have many other proteins present (such as lysates) may require preclearing to prevent interference in later IP and WB procedures. Recombinant protein samples require pre-clearing more often than serum samples.
How can I enrich/concentrate my lysate for a low expression of a protein of interest?
The immunoprecipitation procedure can be repeated several times to yield a more concentrated protein solution.
What is the recommended lysis buffer for TrueBlot® products?
The optimal lysis buffer will depend on your sample type. See protocol for buffer recommendations. Generally, 1X RIPA is used to lyse samples.
My target protein has the same MW as a heavy/light chain. How can I be sure that the band is the target protein and not a heavy/light chain?
Be sure to include a positive control for the primary antibody in your experiment and check that the sample is fully reduced.
What is the average size of TrueBlot® magnetic/agarose beads?
The beads are roughly 0.5 μm in diameter.
Why can; Rabbit Ig IP beads, which are available in either a magnetic or agarose format. Please see individual TrueBlot® Rabbit product pages for additional details.
Can I use Protein A or Protein G beads for the IP step in IP; Ig IP beads, which are available in either agarose or magnetic format.
Adherent Cell Lysis Protocol
Bifunctional Cross-Linking Protocol
Biotin Binding Assay Protocol
Chromatin Immunoprecipitation (ChIP) Protocol
ELISA Protocol
Fluorescent Western Blotting Protocol
Histone Immunoblotting Protocol
Immunocytochemistry (ICC) Protocol
Immunofluorescence (IF) Protocol
Immunoprecipitation (IP) Protocol
In-Cell Western (ICW) Protocol
IP-WB with TrueBlot® Protocol
Multi-Lysate Western Blotting Protocol
Nuclear & Cytoplasmic Extract Protocol
Nucleosome ChIP Protocol
Peptide Competition Assay (PCA) Protocol
Re-ChIP Protocol
Sandwich ELISA Protocol for Collagen
Suspension Cultured Cell Lysis Protocol
Western Blotting (WB) Protocol
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
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