Myeloperoxidase (MPO) Activity Assay Kit
Referencia E-BC-K074-M-96T
embalaje : 96T
Marca : Elabscience
Contact local distributor :
Teléfono : +1 850 650 7790
| Detection Principle | Myeloperoxidase reduces hydrogen peroxide to a complex. The complex react with o-dianisidine (as hydrogen donor) to produce a yellow product which has a maximum absorption peak at 460 nm. The activity of MPO can be calculated indirectly by measuring the OD value at 460 nm. |
| Synonyms | MPO |
| Sample Type | Serum,Plasma,Animal tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (450-470 nm, optimum wavelength: 460 nm) |
| Research Area | Oxidative Stress |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 19.42 U/L |
| Detection Range | 19.42-893.31 U/L |
| Precision | inter-assay CV: 7.3% | intra-assay CV: 5.4% |
| Assay Time | 1 h 10 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 1 |
| Human plasma | 1 |
| Human milk | 1 |
| Cell culture supernatant | 1 |
| Rat serum | 1 |
| Rat plasma | 1 |
| 5% Rat kidney tissue homogenate | 2-3 |
| 5% Rat spleen tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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